| 广西巴马小型猪ApoE基因外显子2片段的多态性分析 |
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| 引用本文： | 黄玥萌,韦玲静,蒋钦杨,兰干球,郭亚芬.广西巴马小型猪ApoE基因外显子2片段的多态性分析[J].实验动物科学,2014(3):42-45. |
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| 作者姓名： | 黄玥萌 韦玲静 蒋钦杨 兰干球 郭亚芬 |
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| 作者单位： | 广西大学动物科学技术学院,南宁530004 |
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| 基金项目： | 国家自然科学基金项目（No.81360135）;广西科学研究与技术开发计划项目（桂科攻1347003-2）;广西自然科学基金项目（No.2013GXNSFAA019187） |
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| 摘 要： | 目的克隆广西巴马小型猪载脂蛋白E(ApoE)基因外显子2片段并分析该片段的多态性,为建立广西巴马小型猪动脉粥样硬化模型奠定基础。方法以广西巴马小型猪基因组DNA为模板,PCR扩增ApoE基因片段,应用PCR-SSCP方法分析其多态性。结果克隆获得长237 bp的ApoE基因外显子2片段,测序结果比对参考序列同源性为100%。PCR-SSCP分析发现在该片段的229位点存在多态性,表现为AA型、AT型和TT型3种基因型。结论成功克隆广西巴马小型猪ApoE基因外显子2片段,该片段存在多态性,有AA、AT和TT 3种基因型,基因型频率分别为0.302、0.489和0.209。
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| 关 键 词： | 广西巴马小型猪 载脂蛋白E PCR-SSCP |
Polymorphism of Exon2 Sequence in ApoE Gene of Guangxi Bama Mini Pig |
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| HUANG Yue-meng,WEI Ling-jing,JIANG Qin-yang,LAN Gan-qiu,GUO Ya-fen.Polymorphism of Exon2 Sequence in ApoE Gene of Guangxi Bama Mini Pig[J].Shiyan Dongwu Kexue,2014(3):42-45. |
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| Authors: | HUANG Yue-meng WEI Ling-jing JIANG Qin-yang LAN Gan-qiu GUO Ya-fen |
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| Institution: | 1.Animal Science and Technology College of Guangxi University, Nanning 530004, China;) |
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| Abstract: | Objective Cloning and analysis of ApoE gene sequence in Guangxi Bama mini-pig were carried out to establish the basic genetic data for Atherosclerosis model in Guangxi Bama mini-pig.Method A 237bp sequence of the ApoE gene was cloned,polymorphism analysis was performed using PCR-SSCP.Result The PCR-SSCP analysis found that in the 229 loci existed polymorphisms of ApoE gene fragments,characterized by AA,the AT and TT type three genotypes.And the cloned sequencing was 100％ homology compared with Sus Scrofa.Conclusion ApoE gene sequence of Guangxi miniature pig was successfully cloned.And the gene fragment showed AA,AT and TT three genotypes,and the genotype frequences were 0.302,0.489 and O.209. |
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| Keywords: | Guangxi Bama mini-pig ApoE PCR-SSCP analysis |
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