广西巴马小型猪 ApoE 基因外显子 2 片段的多态性分析

摘要: 目的 克隆广西巴马小型猪载脂蛋白 E( ApoE) 基因外显子 2 片段并分析该片段的多态性，为建立广西巴马 小型猪动脉粥样硬化模型奠定基础。方法 以广西巴马小型猪基因组 DNA 为模板，PCＲ 扩增 ApoE 基因片段，应 用 PCＲ-SSCP 方法分析其多态性。结果 克隆获得长 237 bp 的 ApoE 基因外显子 2 片段，测序结果比对参考序列同 源性为 100% 。PCＲ-SSCP 分析发现在该片段的 229 位点存在多态性，表现为 AA 型、AT 型和 TT 型 3 种基因型。结 论 成功克隆广西巴马小型猪 ApoE 基因外显子 2 片段，该片段存在多态性，有 AA、AT 和 TT 3 种基因型，基因型频 率分别为 0. 302、0. 489 和 0. 209。

Polymorphism of Exon2 Sequence in ApoE Gene of Guangxi Bama Mini Pig

Objective Cloning and analysis of ApoE gene sequence in Guangxi Bama mini-pig were carried out to establish the basic genetic data for Atherosclerosis model in Guangxi Bama mini-pig.Method A 237bp sequence of the ApoE gene was cloned,polymorphism analysis was performed using PCR-SSCP.Result The PCR-SSCP analysis found that in the 229 loci existed polymorphisms of ApoE gene fragments,characterized by AA,the AT and TT type three genotypes.And the cloned sequencing was 100％ homology compared with Sus Scrofa.Conclusion ApoE gene sequence of Guangxi miniature pig was successfully cloned.And the gene fragment showed AA,AT and TT three genotypes,and the genotype frequences were 0.302,0.489 and O.209.