鸡传染性法氏囊病的RT-PCR诊断方法的研究

在研究鸡法氏囊病毒(IBDV)的A片断cDNA结构的基础上,建立了RT-PCR早期诊断技术.结果表明,使用PrimerDesign引物设计软件,在VP5和VP2的重叠基因区设计的一对引物具有非常高的同源性和保守性.采用的四种IBDV的标准毒株、一种野毒株和一例病鸡标本通过此引物进行RT-PCR检测均得到了明显的阳性扩增结果.而两种其他鸡病病毒和一例健康鸡的法氏囊组织扩增均为阴性结果.

Diagnosis for IBDV of Chickens

The primers designed by PrimerDesign software in the overlapping area of VP5 and VP2 where the sequence is highly homologous are applicable to various strain of IBDV which are verified through scanning in the Internet and experiments. Four standard virus strains, one wild strain and one IBDV strain in the bursa of Fabriclus of diseased chickens are amplified successfully by the primers. Two other viruses which can result in other chicken diseases and one healthy chicken's bursa of Fabriclus show the negative results. The process of PCR is to operate and lower down the cost of the process. All of the above mentioned provide a new technology and diagnosis reagent for the earlier diagnosis and the prevention of IBDV.