K252a: A new blocker of the cell-cycle at G1 phase in a human hepatoma cell line |
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| Authors: | T. Nakayama Y. Hashimoto Y. Kaneko M. Yoshida T. Beppu K. Ohmi S. Yamashita Y. Nonomura K. Kurokawa |
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| Affiliation: | (1) The First Department of Internal Medicine, Faculty of Medicine, the University of Tokyo, Hongo 7-3-1, Bunkyo-ku, 113 Tokyo, (Japan);(2) The First Department of Pharmacology, Faculty of Medicine, the University of Tokyo, Hongo 7-3-1, Bunkyo-ku, 113 Tokyo, (Japan);(3) Department of Agricultural Chemistry, Faculty of Agriculture, the University of Tokyo, Hongo 7-3-1, Bunkyo-ku, 113 Tokyo, (Japan) |
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| Abstract: | The administration of 200 nM K252a to HuH7 suppressed the proliferation of the cells almost completely. The uptake of [3H]thymidine was inhibited, and flow cytometry revealed only one peak at 2C on day 3 after treatment with 100 nM K252a. The expression of proto-oncogene c-myc was not reduced. Despite the blockage at G1, both the size of the cells and the amount of cell protein had increased by 4 times by day 3 after treatment with K252a, while the cells secreted albumin and  -fetoprotein into the medium as usual. These results show that K252a can increase the cell size of HuH7 without losing its function by blocking the cell cycle at G1 phase. |
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| Keywords: | K252a G1 block cell size c-myc albumin HuH7 |
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