| 水提分离钝顶螺旋藻藻蓝蛋白及其稳定性研究 |
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| 引用本文： | LIU Yang,王雪青,ZHAO Pei,庞广昌.水提分离钝顶螺旋藻藻蓝蛋白及其稳定性研究[J].天津师范大学学报(自然科学版),2008,28(3):11-14. |
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| 作者姓名： | LIU Yang 王雪青 ZHAO Pei 庞广昌 |
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| 作者单位： | 天津商业大学,生物技术与食品科学学院,天津,300134 |
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| 基金项目： | 天津市重大科技攻关项目
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天津市自然科学基金重点项目 |
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| 摘 要： | 应用水提法分离钝顶螺旋藻藻蓝蛋白后,考察了螺旋藻细胞破碎液的藻液比、盐离子浓度、pH和环境温度等对藻蓝蛋白分离的影响,并对藻蓝蛋白的耐热性能及耐酸碱性能进行了研究.结果表明：用藻液比为5 g/L及50%（NH4）2SO4进行盐析沉淀,提取藻蓝蛋白的收率可达62.4 mg/g藻粉;螺旋藻藻蓝蛋白在常温时保持稳定,在较高温度时稳定性下降;在中性溶液环境时,藻蓝蛋白的稳定性良好,偏酸或偏碱环境均改变螺旋藻藻蓝蛋白的性能.
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| 关 键 词： | 螺旋藻 藻蓝蛋白 水提法 收率 |
On the stability and separation of c-phycocyanin from Spirulina platensis by water extraction |
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| LIU Yang,WANG Xueqing,ZHAO Pei,PANG Guangchang.On the stability and separation of c-phycocyanin from Spirulina platensis by water extraction[J].Journal of Tianjin Normal University(Natural Science Edition),2008,28(3):11-14. |
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| Authors: | LIU Yang WANG Xueqing ZHAO Pei PANG Guangchang |
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| Institution: | LIU Yang , WANG Xueqing , ZHAO Pei, PANG Guangchang(1. Tianjin Key Laboratory of Food Biotechnology;2. College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China) |
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| Abstract: | The effects of solvent-biomass ratio of the Spirulina cellular extract,（NH4）2SO4 solutions with different concentration,pH and temperature on the separation of c-phycocyanin from the supernatant of Spirulina platensis by water extraction were studied.The stabilities of c-phycocyanin in the conditions of different temperature and pH were studied also.The results showed that the yield of c-phycocynain reached 62.4 mg/g by salting-out precipitation with solvent-biomass ratio of 5 g/L and 50%（NH4）2SO4.In the solution with pH=7 and in the room-temperature the stability of c-phycocyanin was well but reduced in higher temperature,as well as in weak acid or weak base environment. |
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| Keywords: | Spirulina c-phycocyanin water extraction yield |
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