人血清免疫球蛋白G的分离及其重、轻链的拆解

用DEAE-纤维素离子交换柱色谱法从人血清中能分离出IgG。以β-巯基乙醇还原和过甲酸氧化两种方法打开IgG分子链间的二硫键,拆离重链和轻链,获得用于免疫试验的特异性重链。

ISOLATION OF HUMAN SERUM IgG AND CLEAVAGE OF ITS HEAVY AND LIGHT CHAINS

IgG was isolated from human serum by DEAE-Cellulose ion exchange chromatography.The disulfide bonds in the IgG chains were cledved by β-mereaptothanol (reduction) and perfomic acid(oxidation) The isolation of heavy and light chains in the IgG was performed by Sephadex G-100 Column Chromatography.