| Monoclonal Antibodies Recognizing HIV-1 gp41 Could Inhibit Env-Mediated Syncytium Formation |
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| 引用本文： | 张耿 陈应华. Monoclonal Antibodies Recognizing HIV-1 gp41 Could Inhibit Env-Mediated Syncytium Formation[J]. 清华大学学报, 2005, 10(4): 512-516. DOI: 10.1016/S1007-0214(05)70109-7 |
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| 作者姓名： | 张耿 陈应华 |
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| 作者单位： | [1]Laboratory of Immunology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China [2]Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing 100084, China |
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| 基金项目： | Supported by the National Natural Science Foundation of China（No. 30270286） |
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| 摘 要： | Some monoclonal antibodies （mAbs） could inhibit infection by HIV-1. In this study, four mAbs against HIV-1 gp41 were prepared in mice. All four mAbs could bind to the recombinant soluble gp41 and recognize the native envelope glycoprotein gp160 expressed on the HIV-Env^＋ CHO-WT cell in flow cytometry analysis. Interestingly, the results show that all four mAbs purified by affinity chromatography could inhibit HIV-1 Env-mediated membrane fusion （syncytium formation） by 40%-60% at 10 μg/mL, which implies potential inhibitory activities against HIV-1.
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| 关 键 词： | HIV 爱滋病病毒 治疗方法 单克隆技术 |
| 收稿时间： | 2005-01-11 |
| 修稿时间： | 2005-01-11 |
Monoclonal Antibodies Recognizing HIV-1 gp41 Could Inhibit Env-Mediated Syncytium Formation |
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| Geng Zhang, ,#xf; ,#xf;,Yinghua Chen, ,#x;,#xe;,#xe;. Monoclonal Antibodies Recognizing HIV-1 gp41 Could Inhibit Env-Mediated Syncytium Formation[J]. Tsinghua Science and Technology, 2005, 10(4): 512-516. DOI: 10.1016/S1007-0214(05)70109-7 |
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| Authors: | Geng Zhang, ,Yinghua Chen, &#x |
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| Affiliation: | aLaboratory of Immunology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;bProtein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing 100084, China |
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| Abstract: | Some monoclonal antibodies (mAbs) could inhibit infection by HIV-1. In this study, four mAbs against HIV-1 gp41 were prepared in mice. All four mAbs could bind to the recombinant soluble gp41 and recognize the native envelope glycoprotein gp160 expressed on the HIV-Env+ CHO-WT cell in flow cytometry analysis. Interestingly, the results show that all four mAbs purified by affinity chromatography could inhibit HIV-1 Env-mediated membrane fusion (syncytium formation) by 40%-60% at 10 μg/mL, which implies potential inhibitory activities against HIV-1. |
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| Keywords: | HIV-1 monoclonal antibody cell fusion |
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