High and low affinity transport ofL-arginine in rat brain synaptosomes |
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Authors: | C H Tan F H Ng |
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Institution: | (1) Department of Biochemistry, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 0511 Singapore, (Singapore) |
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Abstract: | The uptake ofL-arginine into purified rat brain synaptosomes was investigated with respect to time and various concentrations ofL-3H] arginine. Specific uptake was found to be linear with time for up to 5 min of incubation at 37°C. Electrolytes, including sodium chloride, potassium chloride, magnesium chloride and calcium chloride, inhibited uptake of 3 ML-arginine, and the inhibitory effect increased with increased electrolyte concentration under constant osmolarity. It was found thatL-arginine was transported into synaptosomes by two uptake components — a high affinity component (3.5 M) and a low affinity component (100 M). These two components were similar to the Ly+ system because of their extreme sensitivity to inhibition byL-lysine andL-ornithine but were distinguishable from each other by kinetic analysis of the uptake data and by their relative sensitivity to inhibition by several amino acids. |
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Keywords: | L-arginine" target="_blank">L-arginine L-arginine transport" target="_blank">L-arginine transport L-arginine uptake" target="_blank">L-arginine uptake rat brain synaptosomes nerve endings amino acid uptake transport systems |
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