| 丙戊酸诱导多药耐药白血病细胞HL-60／ADR凋亡的研究 |
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| 引用本文： | 龙家茹,廖丹琼,杨赣军. 丙戊酸诱导多药耐药白血病细胞HL-60／ADR凋亡的研究[J]. 井冈山学院学报, 2009, 30(4): 72-74 |
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| 作者姓名： | 龙家茹 廖丹琼 杨赣军 |
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| 作者单位： | 龙家茹,杨赣军(井冈山大学,医学院,江西,吉安,343000);廖丹琼(井冈山大学,护理学院,江西,吉安,343000) |
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| 摘 要： | 目的研究丙戊酸对多药耐药白血病细胞HL-60／ADR的抑制作用及其机制。方法采用MTT比色试验观察丙戊酸对多药耐药细胞HL-60／ADR的抑制作用。采用光镜技术观察细胞形态结构的改变，利用流式细胞术检测细胞凋亡率。结果（1）丙戊酸能明显抑制HL-60／ADR细胞的生长，药物浓度相当于生药32．65mg／mL时，即具有显著的抑制作用，抑制率呈剂量和时间依赖性，半数抑制浓度0c．50）相当于生药66．44mg／mL。（2）形态学改变呈典型的凋亡特征。（3）丙戊酸能诱导多药耐药细胞HL-60／ADR凋亡，凋亡率也呈时间和剂量的依赖性。结论丙戊酸对多药耐药细胞HL-60／ADR的生长具有极强的抑制作用，诱导其凋亡是其机制之一。
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| 关 键 词： | 丙戊酸 多药耐药 HL-60／ADR 凋亡 |
Effect of valproic acid on inducing apoptosis of leukemic cell line HL-60/ADR with multidrug resistance |
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| LONG Jia-ru,LIAO Dan-qiong,YANG Gan-jun. Effect of valproic acid on inducing apoptosis of leukemic cell line HL-60/ADR with multidrug resistance[J]. Journal of Jinggangshan University, 2009, 30(4): 72-74 |
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| Authors: | LONG Jia-ru LIAO Dan-qiong YANG Gan-jun |
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| Affiliation: | 1.School of Medicine;Jinggangshan University;Ji'an 343000;China;2.School of Nursing;China |
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| Abstract: | Objective:To study the effect of Valproic acid on inducing apoptosis of leukemic cell line HL-60 / ADR with multidrug resistance.Methods:The depressant effect of Valproic acid on multidrug resistance leukemic cell line HL-60 / ADR were measured by MTT method,the changes of cellular morphology were observed under light microscope.The apoptosis rates were detected by flow cytometry.Results:Valproic acid could significantly inhibit the leukemic cell proliferation in a dose-and time-dependent manner,the minimal... |
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| Keywords: | HL-60/ADR |
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