| 黄花倒水莲（Polygala fallax）多糖含量的测定 |
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| 引用本文： | 黄翔,王晓平,黎建玲.黄花倒水莲（Polygala fallax）多糖含量的测定[J].玉林师范学院学报,2013(5):65-68. |
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| 作者姓名： | 黄翔 王晓平 黎建玲 |
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| 作者单位： | [1]玉林师范学院体育学院,广西玉林537000 [2]玉林师范学院生命科学与技术学院,广西玉林537000 |
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| 基金项目： | 广西教育厅2011年科研基金资助项目（200103YB121）;玉林师范学院高层次人才科研启动基金项目（G20120017）. |
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| 摘 要： | 目的建立黄花倒水莲多糖含量的测定方法．方法以葡萄糖为对照品，采用葸酮-硫酸法在625nm处测定多糖含量．结果葡萄糖对照品溶液在O．02mg／mL-0．12mg／mL范围内呈良好曲线关系，线性回归方程为：A=11．8926C-0．1363，RL=O．9986，平均加样回收率为100．4％，RSD为1．69％．结论该方法操作方便、快速、重现性好，可用于黄花倒水莲多糖含量的测定．
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| 关 键 词： | 黄花倒水莲 葸酮-硫酸法 多糖 |
Determination of Polysaccharide in Polygala fallax Hemsl |
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| HUANG Xiang,WANG Xiao-ping,LI Jian-ling.Determination of Polysaccharide in Polygala fallax Hemsl[J].Journal of Yulin Teachers College,2013(5):65-68. |
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| Authors: | HUANG Xiang WANG Xiao-ping LI Jian-ling |
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| Institution: | 1. College ofP. E., Yulin Normal Univresity, Yulin, Guangxi 537000; 2. College of Life Science ＆ Technology, Yulin Normal University, Yulin, Guangxi 537000 ) |
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| Abstract: | Objective： To establish the method for determination the polysaccharide in Polygala fallax Hemsl. Methods： With glucose as reference substance, and use Anthrone-sulfuric acid methods to determination the polysaccharide in 625nm. Results： The glucose control solution had a good linearity in the range of 0.02mg/mL- 0.12mg/mL. The linear regression standard curve was A= 11.8926C-0.1363, R2=0.9986. The mean recovery rate was 100.4% and the RSD was1.69%. Conclusion： The rapid method is convenient and has good reproducibility. It can be used for determination of the polysaccharide in Polygala fallax Hemsl. |
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| Keywords: | Polygala fallax Hemsl Anthrone-sulfuric acid methods polysaccharide |
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