| PF18-3,a 35-ku molecule, expresses specifically on apoptotic subset of mouse thymocytes |
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| 引用本文： | WANG Hong, LI Yan, PANG Xuewen &CHEN Weifeng（Key Laboratory of Medical Immunology, Ministry of Health, and Department of Immunology,Beijing Medical University, Beijing 100083, China）Correspondence should be addressed to Chen Weifeng. PF18-3,a 35-ku molecule, expresses specifically on apoptotic subset of mouse thymocytes[J]. 科学通报(英文版), 2000, 45(1): 358-362 |
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| 作者姓名： | WANG Hong, LI Yan, PANG Xuewen & CHEN Weifeng（Key Laboratory of Medical Immunology, Ministry of Health, and Department of Immunology,Beijing Medical University, Beijing 100083, China）Correspondence should be addressed to Chen Weifeng |
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| 摘 要： |
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| 关 键 词： | thymocytes cell apoptosis monoclonal antibody mediator.▲ |
PF18-3,a 35-ku molecule, expresses specifically on apoptotic subset of mouse thymocytes |
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| Abstract: | PF18-3monoclonal antibody (mAb), one of the rat mAbs against mouse thymic stromal cells (MTSC),has been found to inhibit thymocyte apoptosis induced by a mouse thymic dendritic cellline, MTSC4, in previous co-culture study. The aim of this research is to investigate thecharacter of PF18-3 mAb recognized molecule ( PF18-3 molecule) and its role inMTSC4-induced thymocyte apoptosis. The characterization of PF18-3 molecule expression hasbeen conducted by FACS analysis. PF18-3 molecules have been found to express on MTSC4 aswell as on Con A activated but not freshly isolated thymocytes. Up-regulated expression ofPF18-3 molecules has been also observed on thymocytes after being co-cultured with MTSC4for 48 h. The results from FACS analyses by PI staining for detecting apoptosis-relatedhypodiploid and by PF18-3 mAb staining reveal that PF18-3 molecules expresss specificallyon the apoptotic subgroup of thymocytes with high hypodiploid content. The PF18-3 moleculeexpressed on apoptotic thymocytes with 35 ku of molecular weight, identified byimmunoprecipitation and western blotting, is thus likely to be a molecule involved inthymocyte apoptosis. |
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