PF18-3,
a 35-ku molecule, expresses specifically on apoptotic subset of mouse thymocytes |
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作者姓名: | WANG Hong LI Yan PANG Xuewen &
CHEN Weifeng |
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摘 要: |
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关 键 词: | thymocytes cell apoptosis monoclonal antibody mediator.▲ |
PF18-3,
a 35-ku molecule, expresses specifically on apoptotic subset of mouse thymocytes |
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Abstract: | PF18-3
monoclonal antibody (mAb), one of the rat mAbs against mouse thymic stromal cells (MTSC),
has been found to inhibit thymocyte apoptosis induced by a mouse thymic dendritic cell
line, MTSC4, in previous co-culture study. The aim of this research is to investigate the
character of PF18-3 mAb recognized molecule ( PF18-3 molecule) and its role in
MTSC4-induced thymocyte apoptosis. The characterization of PF18-3 molecule expression has
been conducted by FACS analysis. PF18-3 molecules have been found to express on MTSC4 as
well as on Con A activated but not freshly isolated thymocytes. Up-regulated expression of
PF18-3 molecules has been also observed on thymocytes after being co-cultured with MTSC4
for 48 h. The results from FACS analyses by PI staining for detecting apoptosis-related
hypodiploid and by PF18-3 mAb staining reveal that PF18-3 molecules expresss specifically
on the apoptotic subgroup of thymocytes with high hypodiploid content. The PF18-3 molecule
expressed on apoptotic thymocytes with 35 ku of molecular weight, identified by
immunoprecipitation and western blotting, is thus likely to be a molecule involved in
thymocyte apoptosis. |
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