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HPLC法测定金匮肾气丸中马钱苷、芍药苷的含量
引用本文:李文兰,赵稷,王晓冬,扈正婷. HPLC法测定金匮肾气丸中马钱苷、芍药苷的含量[J]. 哈尔滨商业大学学报(自然科学版), 2008, 24(1): 12-15
作者姓名:李文兰  赵稷  王晓冬  扈正婷
作者单位:哈尔滨商业大学,生命科学与环境科学研究中心,哈尔滨,150076
基金项目:黑龙江省科技厅科技攻关项目 , 黑龙江省教育厅振兴老工业基地项目 , 黑龙江省教育厅科学技术研究项目
摘    要:建立一种方法用于同时测定金匮肾气丸中马钱苷、芍药苷的含量.应用高效液相色谱法测定,分离条件Symmetry C18柱(4.6min×250mm,5μm),乙腈-1%冰醋酸溶液(14:86)为流动相,流速为1mL/min,柱温为30℃、马钱苷、芍药苷线性范围分别为0.16~1.6μg(r=0.9997),0.18~1.8μg(r=0.9999),平均加样回收率分别为99.13%(RSD=1.43%),99.02%(RSD=2.17%)、方法简便,快速,可靠,为金匮肾气丸进一步研究奠定基础.

关 键 词:金匮肾气丸  马钱苷  芍药苷  HPLC
文章编号:1672-0946(2008)01-0012-04
收稿时间:2007-07-29
修稿时间:2007-07-29

Determination of loganin and paeoniflorin in Jinkuishenqi pill by HPLC
LI Wen-lan,ZHAO Ji,WANG Xiao-dong,HU Zheng-ting. Determination of loganin and paeoniflorin in Jinkuishenqi pill by HPLC[J]. Journal of Harbin University of Commerce :Natural Sciences Edition, 2008, 24(1): 12-15
Authors:LI Wen-lan  ZHAO Ji  WANG Xiao-dong  HU Zheng-ting
Affiliation:LI Wen-lan, ZHAO Ji, WANG Xiao-dong, HU Zheng-ting ( Center of Research on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China)
Abstract:To establish an accurate and practical HPLC method for the simultaneous of loga- nin and paeoniflorin in Jinkuishenqi pill. Chromatographic separation is operated on a waters Symmetry C18 column (4.6mm × 250mm,5 μm) with mobile phase of acetonitrile - 0.1% ice acetic acid at flow rate of 1.0mL/min and a column temperature of 30 ℃. The calibration curves of loganin and paeoniflorin are linear over the ranges of 0.16 - 1.6 μg( r = 0.999 7) and 0.18 - 1.8 μg ( r - 0. 999 9), and the average recoveries are 99.13% ( RSD = 1.43 % ) and 99.02% (RSD =2.17% ) ,respectively. This method is simple, rapid, reliable and can used for groundwork for Jinkuishenqi pill's further investigation.
Keywords:Jinkuishenqi pill   loganin   paeoniflorin   HPLC
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