| 中国大鲵(Andrias davidianus)二十四种组织器官的碱性磷酸酶(AKP)初步分析 |
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| 引用本文： | 沈国民,辛泽华,乔志刚,徐存拴. 中国大鲵(Andrias davidianus)二十四种组织器官的碱性磷酸酶(AKP)初步分析[J]. 河南科学, 2003, 21(3): 283-285 |
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| 作者姓名： | 沈国民 辛泽华 乔志刚 徐存拴 |
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| 作者单位： | 1. 河南省生物工程重点实验室,河南,新乡,453002
2. 河南师范大学生命科学学院,河南,新乡,453002 |
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| 基金项目： | 国家自然科学基金(3025001) |
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| 摘 要： | 用酶的原位复性电泳方法分析了中国大鲵二十四种组织器官的碱性磷酸酶。结果表明,二十四种组织器官中都有碱性磷酸酶存在,其中230和200 KD的碱性磷酸酶占优势;不同组织器官的碱性磷酸酶活性有差异,心脏中活性最强,眼球中活性最弱;在同一组织中,230和200 KD的碱性磷酸酶活性也有差异。另外,在肾脏和心脏中还含有一种250 KD的碱性磷酸酶,推测与器官的功能有关。
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| 关 键 词： | 中国大鲵 碱性磷酸酶 酶原位复性电泳 组织器官 |
| 文章编号： | 1004-3918(2003)03-0283-03 |
| 修稿时间： | 2003-03-15 |
Analysis of alkaline phosphatase (AKP) in twenty-four kinds of tissues and organs of Andrias davidianus |
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| SHEN Guomin,XIN Ze-hua,QIAO Zhi-gang,XU Cun-shuan. Analysis of alkaline phosphatase (AKP) in twenty-four kinds of tissues and organs of Andrias davidianus[J]. Henan Science, 2003, 21(3): 283-285 |
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| Authors: | SHEN Guomin XIN Ze-hua QIAO Zhi-gang XU Cun-shuan |
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| Abstract: | AKP in twenty-four kinds of tissues and organs of Andrias davidianus were analyzed by the renatured electrophoresis. The results indicated that AKP exist in all tissues and organs of Andrias davidianus, which were mainly 230 and 200 KD. Activity of AKP in different tissues and organs was different, and these were strongest in heart and weakest in eyes. In the same tissues and organs, two kinds of AKP activities were different. In addition, a kind of 250 KD AKP was found in kidney and heart, whick probably was involved in functions of these organs. |
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| Keywords: | Andrias davidianus alkaline phosphatase tissues and organs renatured electrophoresis |
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