| 聚合酶链反应检测石蜡切片中金黄色葡萄球菌DNA的研究 |
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| 引用本文： | 高正琴,邢华,孙怀昌,李厚达. 聚合酶链反应检测石蜡切片中金黄色葡萄球菌DNA的研究[J]. 实验动物科学, 2004, 21(1): 51-52 |
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| 作者姓名： | 高正琴 邢华 孙怀昌 李厚达 |
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| 作者单位： | 扬州大学比较医学中心,扬州,225009 |
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| 基金项目： | 国家“十五”科技攻关计划“国家遗传工程小鼠资源库的建立”(项目编号 :2 0 0 1BA70 113 ) |
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| 摘 要： | 目的 探讨聚合酶链反应技术 (PCR)在检测石蜡包埋组织中金黄色葡萄球菌DNA的应用及价值。方法 利用PCR技术 ,对 55例福尔马林固定、石蜡包埋小鼠组织中SA DNA进行了检测。结果 55例石蜡包埋的小鼠组织中均检测到SA DNA ,这与临床病理诊断为金黄色葡萄球菌 (S .aureus)感染相一致。结论 PCR技术可用于检测石蜡包埋组织中的SA DNA片段 ,为鼠S .aureus感染的回顾性分析提供了有效手段
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| 关 键 词： | 聚合酶链反应 金黄色葡萄球菌 石蜡包埋组织标本 |
| 文章编号： | 1006-6179(2004)01-0051-02 |
| 收稿时间： | 2004-03-25 |
| 修稿时间： | 2003-03-06 |
Detection of Staphylococcus aureus DNA in Paraffin-embedded Specimens by Polymerase Chain Reaction |
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| GAO Zheng_qin,XING Hua,SUN Huai_chang,LI Hou_da. Detection of Staphylococcus aureus DNA in Paraffin-embedded Specimens by Polymerase Chain Reaction[J]. Laboratory Animal Science, 2004, 21(1): 51-52 |
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| Authors: | GAO Zheng_qin XING Hua SUN Huai_chang LI Hou_da |
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| Abstract: | Objective To pvovide an additional diff erential diagnostic method for pathological diagnosis of Staphylococcus aureus (S.aureus) infection in formalin_fixed and paraffin_embedded tissue samples b y Pol ymerase Chain Reaction(PCR). Methods 55 tissue samples of formalin_ fixed and paraffin_embedded tissue samplex of laboratory mice pathologically dia gnosed as S.aureus inflammation were examined by PCR. Results Among them, 55 tissue samples all showed positive results:the specific bands of 678bp were present in the agarose gel. Conclusion The results showed the PCR assay developed is a reliable method and very useful for retrospective stud y of S.aureus infection on the molecular level. |
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| Keywords: | Polymerase Chain Reaction(PCR) %Staphylococcus aureus% Paraffin_embedded tissue samples |
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