| 怀牛膝愈伤组织诱导及分化的研究 |
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| 引用本文： | 李明军,李萍,洪森荣,张宝华,柳俊,谢从华.怀牛膝愈伤组织诱导及分化的研究[J].河南师范大学学报(自然科学版),2005,33(4):118-121. |
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| 作者姓名： | 李明军 李萍 洪森荣 张宝华 柳俊 谢从华 |
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| 作者单位： | 河南师范大学,生命科学学院,河南,新乡,453007;华中农业大学,园艺植物生物学教育部重点实验室,武汉,430070;河南师范大学,生命科学学院,河南,新乡,453007;河南师范大学,生命科学学院,河南,新乡,453007;上饶师范学院,生命科学系,江西,上饶,334001;温县农业科学研究所,河南,温县454881;华中农业大学,园艺植物生物学教育部重点实验室,武汉,430070 |
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| 基金项目： | 河南省科技攻关项目(0524420001) |
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| 摘 要： | 对怀牛膝叶片和茎段愈伤组织的诱导及分化进行了研究.结果表明:KT 0.5 mg/L+2,4-D 1.5 mg/L对叶片愈伤组织的诱导效果最好,KT 0.5 mg/L+NAA 1.5 mg/L对茎段愈伤组织的诱导效果最好,出愈率均达100%;KT 0.5 mg/L+NAA 1.5 mg/L有利于叶片形成的愈伤组织分化为根,6-BA 0.5 mg/L+NAA 1.5 mg/L则有利于茎段形成的愈伤组织分化为芽.
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| 关 键 词： | 怀牛膝 愈伤组织 再分化 根 芽 |
| 文章编号： | 1000-2367(2005)04-0118-04 |
| 收稿时间： | 2005-01-20 |
| 修稿时间： | 2005年1月20日 |
The Study on Callus Induction and Redifferentiation Of Achyranthes Bidentata BL. |
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| LI Ming-jun,LI Ping,HONG Sen-rong,ZHANG Bao-hua,LIU Jun,XIE Cong-hua.The Study on Callus Induction and Redifferentiation Of Achyranthes Bidentata BL.[J].Journal of Henan Normal University(Natural Science),2005,33(4):118-121. |
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| Authors: | LI Ming-jun LI Ping HONG Sen-rong ZHANG Bao-hua LIU Jun XIE Cong-hua |
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| Institution: | 1. College of Life Science, Henan Normal University, Xinxiang 453007, China; 2 . Key Laboratory of Horticultural Plant Biology (Huazhong Agricultural University |
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| Abstract: | The object is to explore the effect of the plant growth substance on the callus induction and redifferentiation of Achyranthes bidentata BL..The explants used in culture were leaves and stems of Achyranthes bidentata BL..Results showed: To the leaf,the best medium induced callus was MS basal medium containing 0.5 mg/L KT and 1.5 mg/L 2,4-D;To the stem,the best was MS basal medium containing 0.5 mg/L KT and 1.5 mg/L NAA;To the induction of root from leaf formative callus,the best was MS basal medium containing 0.5 mg/L KT and 1.5 mg/L NAA;To the induction of bud from stem formative callus,the best was MS basal medium containing 0.5 mg/L 6-BA and 1.5 mg/L NAA. |
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| Keywords: | Achyranthes bidentata BL callus redifferentiation root bud |
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