| 青檀基因组DNA提取方法的优化 |
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| 引用本文： | 张慧,张小平,李晓红,陈文龙. 青檀基因组DNA提取方法的优化[J]. 安徽师范大学学报(自然科学版), 2011, 34(3): 256-259 |
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| 作者姓名： | 张慧 张小平 李晓红 陈文龙 |
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| 作者单位： | 安徽师范大学安徽省重要生物资源保护与利用研究重点实验室,安徽芜湖,241000 |
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| 基金项目： | 国家自然科学基金(30840020;30970292) |
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| 摘 要： | 以青檀(Pteroceltis tatarinowii Maxim.)的叶片为材料,采用改良高盐低pH值法、改良SDS法、改良CTAB法和试剂盒法4种不同方法提取其基因组DNA,并进行电泳分析、质量检测及ISSR引物扩增检测.结果表明,改良CTAB法优于其它方法,提取的DNA质量较好,纯度较高,能满足进一步的实验要求.
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| 关 键 词： | 青檀 基因组DNA DNA提取 |
Study on Extraction of Genomic DNA from Pteroceltis tatarinowii Maxim |
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| ZHANG Hui,ZHANG Xiao-ping,LI Xiao-hong,CHEN Wen-long. Study on Extraction of Genomic DNA from Pteroceltis tatarinowii Maxim[J]. Journal of Anhui Normal University(Natural Science Edition), 2011, 34(3): 256-259 |
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| Authors: | ZHANG Hui ZHANG Xiao-ping LI Xiao-hong CHEN Wen-long |
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| Affiliation: | ZHANG Hui,ZHANG Xiao-ping,LI Xiao-hong,CHEN Wen-long(Key Laboratory for the Conservation and Utilization of Important Biological Resources in Anhui Province,Anhui Normal University,Wuhu 241000,China) |
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| Abstract: | The genomic DNA was isolated from the leaf of Pteroceltis tatarinowii Maxim.by using the improved high salt and low pH method,improved SDS method,improved CTAB method and genomic DNA kit.The quality of the DNA was examined by agarose gel electrophoresis,spectrophotometry and PCR amplification of ISSR.The result showed that the method of improved CTAB was obviously better than the others.The DNA sample obtained by the improved CTAB method was pure and had good quality which could satisfy the need for further... |
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| Keywords: | Pteroceltis tatarinowii Maxim genomic DNA DNA extraction |
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