| 榨菜嫩叶高效分化愈伤组织的诱导及无性系的建立 |
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| 引用本文： | 朱婷,陈晓露,师伟,姜长阳. 榨菜嫩叶高效分化愈伤组织的诱导及无性系的建立[J]. 哈尔滨师范大学自然科学学报, 2007, 23(1): 100-104 |
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| 作者姓名： | 朱婷 陈晓露 师伟 姜长阳 |
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| 作者单位： | 辽宁师范大学 |
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| 基金项目： | 辽宁师范大学教学改革项目(20050101123) |
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| 摘 要： | 本实验研究了榨菜嫩叶的愈伤组织的诱导,愈伤组织的分化,分化苗生根、移栽、扦插所需要的条件,建立起榨菜嫩叶的无性系及快速繁殖技术．结果证明:榨菜嫩叶愈伤组织诱导的理想培养基是MS BA 1．2 mg／L 2,4-D 0．5 mg／L;愈伤组织分化的理想培养基是MS BA0．5 mg／L;生根的理想培养基是I／2MS 0．4～0．8 mg／L;以炉灰渣为试管苗的移栽扦插机制,移栽成活率为85％,扦插成活率为80％．
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| 关 键 词： | 榨菜 无性系 快速繁殖 |
| 修稿时间： | 2006-09-13 |
A HIGH EFFICIENT CALLUS INDUCTION AND ESTABLISHMENT OF REGENERATION CLONE OF BRASSICA JUNEAS VAR.TSATSAI |
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| Zhu Ting,Chen Xiaolu,Shi Wei,Jiang Changyang. A HIGH EFFICIENT CALLUS INDUCTION AND ESTABLISHMENT OF REGENERATION CLONE OF BRASSICA JUNEAS VAR.TSATSAI[J]. Natural Science Journal of Harbin Normal University, 2007, 23(1): 100-104 |
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| Authors: | Zhu Ting Chen Xiaolu Shi Wei Jiang Changyang |
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| Affiliation: | Liaoning Normal University |
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| Abstract: | The conditions were studied forinduction, differentation, radication, transplatation, and graftage of the callus of Brassica juneas var. tsatsai and its regenerative clone was established. MS BA 0. 5 mg/L is suitable for differentiation. 1/2MS IAA 0.4 mg/L is suitable for radication with cinder as medium. Survival ration of 85% for transplatation,of 80% for graftage were observed. |
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| Keywords: | Brassica juneas var. tsatsai Clone Rapid propagation |
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