CE-LIF在氨基甲酸酯类农药研究中的应用

用毛细管电泳激光诱导荧光检测法(CE-LIF)，对灭除威（XMC）、混杀威(2, 3, 5-trimethacarb)和灭虫威(mercaptodimethur)等3种氨基甲酸酯类农药进行了研究.在碱性条件下，氨基甲酸酯类农药水解产生甲胺并与荧光衍生试剂4-氯-7-硝基-2，1，3-苯并二唑(NBD-Cl)发生衍生化反应，产物经毛细管电泳激光诱导荧光检测法测定.对影响水解、衍生以及毛细电泳分离的因素如缓冲液酸度、浓度和衍生试剂的浓度等进行了详细讨论；在最佳条件下，3种农药的检出限分别可达3.0×10^-9、1.0×10^-9和3.0×10^-9 mol/L，并测定了它们的水解速率常数及活化能.该方法不仅可对氨基甲酸酯类农药进行测定，同时也可用于对这类农药的一些理化常数的测定.

Study on carbamate pesticides by capillary electrophoresis with laser-induced fluorescence detection

A sensitive and rapid capillary electrophoresis method with laser-induced fluorescence detection（CE-LIF） was developed for the determination of carbamate pesticides（including（3,5- dimethylphenyl） N-methylcarbamate（XMC）,and mercaptodimethur）.In alkaline solution,carbamate was hydrolyzed to methylamine,which can be derivatized by 4-chloro-7-nitrobenzo-2-oxa- 1,3-diazole（NBD-Cl） and detected by CE-LIF.Several parameters which influence the hydrolysis, derivatization and electrophoresis separation,such as pH,concentration of buffer solution and concentration of NBD-Cl,were investigated.The detection limits of the three carbamates were 3.0×10^-9,1.0×10^-9 and 3.0×10^-9’mol/L,respectively.The proposed method was successfully employed to determine the rate constants and activation energies for carbamates hydrolysis.