| 红酵母细胞通透性研究 |
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| 引用本文： | 李永红,朱登勇,王姣,侯立芬,刘宏民.红酵母细胞通透性研究[J].郑州大学学报(理学版),2011,43(4). |
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| 作者姓名： | 李永红 朱登勇 王姣 侯立芬 刘宏民 |
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| 作者单位： | 1. 郑州大学新药研究开发中心 河南郑州450001;郑州大学药学院 河南郑州450001
2. 郑州大学化学系 河南郑州450001 |
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| 摘 要： | 用插入式超声、反复冻融和有机溶剂法对细胞通透性进行处理.结果表明,在超声功率为750 W,超声时间3s和间隔时间4s的条件下,超声处理75次效果最佳；加入甲苯、氯仿和正己烷等有机溶剂均可提高细胞通透性,其中甲苯的效果最好.
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| 关 键 词： | 红酵母 细胞通透性 超声 反复冻融 有机溶剂 |
Study on the Cellular Permeability of Rhodotorula |
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| LI Yong-hong
,
ZHU Deng-yong
,
WANG Jiao
,
HOU Li-fen
,
LIU Hong-min.Study on the Cellular Permeability of Rhodotorula[J].Journal of Zhengzhou University:Natural Science Edition,2011,43(4). |
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| Authors: | LI Yong-hong ZHU Deng-yong WANG Jiao HOU Li-fen LIU Hong-min |
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| Institution: | LI Yong-hong1,2,ZHU Deng-yong3,WANG Jiao3,HOU Li-fen3,LIU Hong-min1,2 (1.New Drug Research & Development Center,Zhengzhou University,Zhengzhou 450001,China,2.School of Pharmaceutical Sciences,3.Department of Chemistry,China) |
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| Abstract: | Probe ultrasound,repeated freeze thawing and organic solvents were used to increase cellular permeability.It was discovered that the best result was obtained with supersonic power 750 W,supersonic time 3 s,time interval 4 s,and the supersonic times 75.Toluene,chloroform and n-hexane could increase cellular permeability,with toluene the best. |
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| Keywords: | rhodotorula cellular permeability ultrasonic repeated freeze-thaw organic solvent |
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