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Distribution of bone marrow stem cells in large porous polyester scaffolds |
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| Authors: | Zhen Wang Zheng Zhang JunChuan Zhang ZhenJue She JianDong Ding |
| Institution: | 1 Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, Department of Macromolecular Science, Fudan Uni-versity, Shanghai 200433, China; 2 Department of Anatomy, Embryology and Histology, Shanghai Medical School, Fudan University, Shanghai 200032, China |
| Abstract: | This work examined the optimal syringing depth during in vitro cell loading in order to even cell distribution after syringing a drop of cell suspension in cylinder poly(lactide-co-glycolide) (PLGA) porous scaffolds. The scaffolds of 10 mm height and 10 mm diameter were fabricated via room-temperature compression molding & particulate leaching technique based on spherical porogens. In vitro tests were employed for such examinations: a global observation of a cell-loaded scaffold stained by the 3-(4,5-dimeth... |
| Keywords: | biomaterials medical polymer tissue engineering scaffold poly (lactic-co-glycolic acid) (PLGA) cell-loading position |
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