| 固定化青霉素酰化酶的新载体 |
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| 引用本文： | 任玲玲,何静,马润宇,段雪.固定化青霉素酰化酶的新载体[J].北京化工大学学报(自然科学版),2002,29(1):64-67. |
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| 作者姓名： | 任玲玲 何静 马润宇 段雪 |
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| 作者单位： | 北京化工大学可控化学反应科学与技术基础教育部重点实验室,北京,100029 |
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| 基金项目： | 国家自然科学基金;29973004; |
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| 摘 要： | 通过X射线粉末衍射、红外光谱等手段考察了α-磷酸锆层柱材料作为载体固定青霉素酰化酶及其对载体结构影响.结果表明无论是否加入戊二醛固定化酶后,层柱材料的结晶度都下降.酶活性和回收率较高,分别为139.5 nmol/s和28.9%.间歇操作稳定性较好,连续操作10次,酶活性下降24%.
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| 关 键 词： | α-磷酸锆 三乙烯四胺 青霉素酰化酶 插层组装 |
| 修稿时间： | 2001年4月12日 |
A New Carrier for immobilization of penicillin acylase |
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| REN Ling,ling,HE Jing,MA Run,yu,DUAN Xue.A New Carrier for immobilization of penicillin acylase[J].Journal of Beijing University of Chemical Technology,2002,29(1):64-67. |
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| Authors: | REN Ling ling HE Jing MA Run yu DUAN Xue |
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| Institution: | The Key Laboratory of Science &;Technology of Controllable Chemical Reactions, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | Penicillin acylase immobilized on triethylaminetetramine-intercalacated-α-ZrP was prepared and characterized by the techniques of X-ray powder diffraction and infrared spectrophotometry.Immobilization of the enzyme led to a decrease in crystallinity of the host solid whether there was glutaraldehyde. The activity of the immobilized enzyme was 139.5 nmol/s, and decreased by only 24% after reusing 10 times. |
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| Keywords: | ZrP triethylaminetetramine penicillin acylase intercalation |
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