| 人乳头瘤病毒11型E7 DNA疫苗质粒的构建和鉴定 |
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| 引用本文： | 李莉华,黄朝晖,陆英华,刘志辉,任金冬,龚佩娟. 人乳头瘤病毒11型E7 DNA疫苗质粒的构建和鉴定[J]. 江南大学学报(自然科学版), 2004, 3(5): 547-550 |
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| 作者姓名： | 李莉华 黄朝晖 陆英华 刘志辉 任金冬 龚佩娟 |
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| 作者单位： | 苏州大学,附属无锡第四医院,无锡市肿瘤研究所,江苏,无锡,214062 |
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| 基金项目： | 江苏省卫生厅重点开放实验室项目(WK200221)资助课题，无锡市卫生局科研项目(ZD0304)资助课题. |
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| 摘 要： | 目的 构建人乳头瘤病毒11型(HPV11)E7 DNA疫苗质粒．方法 从尖锐湿疣病变组织中提取DNA制备模板，采用聚合酶链反应(PCR)技术扩增HPV11-E7基因，将E7基因经双酶切后定向克隆到pcDNA3．1( )中，测序鉴定．结果从病变组织中扩增出了全长HPV11-E7基因，并正向插入载体pcDNA3．1( )中，经酶切和测序鉴定无误．结论 HPV11-E7 DNA疫苗质粒构建成功，为今后进行防治尖锐湿疣的动物实验和临床实验奠定了基础．
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| 关 键 词： | 人乳头瘤病毒 基因克隆 聚合酶链反应 |
| 文章编号： | 1671-7147(2004)05-0547-03 |
Construction and Identified of a DNA Vaccine Plasmid of Human Papillomavirus Type 11 E 7 Gene |
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| LI Li-hua,HUANG Zhao-hui,LU Ying-hua,LIU Zhi-hui,REN Jin-dong,GONG Pei-juan. Construction and Identified of a DNA Vaccine Plasmid of Human Papillomavirus Type 11 E 7 Gene[J]. Journal of Southern Yangtze University:Natural Science Edition, 2004, 3(5): 547-550 |
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| Authors: | LI Li-hua HUANG Zhao-hui LU Ying-hua LIU Zhi-hui REN Jin-dong GONG Pei-juan |
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| Abstract: | OBJECTIVETo construct DNA vaccine plasmid of human papillomavirus Type 11(HPV11) E7 Gene.METHODSDNA was extracted from biopsy of condyloma tissues and subjected to PCR as template.The gene fragment of HPV11-E7 was amplified by polymerase chain reaction (PCR) and was cloned into the pcDNA 3.1(+).The recombinant plasmid pcDNA 3.1(+)/ HPV11-E7 was identified by restriction endonuclease cleases digestion and sequencing.RESULTSThe amplified fragment from diseased tissue contained the complete HPV 11-E7 gene was inserted into the vector of pcDNA 3.1(+).Sequence in the constructed plasmid was correct.CONCLUSIONThe expression plasmids pcDNA3.1(+)/ HPV11-E7 was constructed successfully, which has paved a way for animal experiment and clinical trial in the future. |
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| Keywords: | human papillomavirus gene cloning polymerase chain reaction |
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