首页 | 本学科首页   官方微博 | 高级检索  
     

牛蛙替代蟾蜍在生理教学实验中的应用研究
引用本文:徐彩云,李天舒,李晓慧,于慧美,郭新荣,张大维,崔佳润. 牛蛙替代蟾蜍在生理教学实验中的应用研究[J]. 实验动物科学, 2021, 38(6): 53-58
作者姓名:徐彩云  李天舒  李晓慧  于慧美  郭新荣  张大维  崔佳润
摘    要:目的 比较牛蛙替代蟾蜍在生理学教学的实验效果,以探索这种实验动物替代的可行性.方法 对牛蛙和蟾蜍分别进行蛙心灌流实验和坐骨神经-腓肠肌刺激收缩实验.结果 蛙心灌流实验中,牛蛙和蟾蜍离体心脏活动在Na+、Ca2+、K+、重酒石酸去甲肾上腺素(NA)、氯化乙酰胆碱(Ach)、乳酸(LA)及碳酸氢钠(NaHCO3)作用的影响...

关 键 词:牛蛙  蟾蜍  生理学实验

Study on the Application of Bullfrog Instead of Toad in Physiology Teaching Experiment
XU Caiyun,LI Tianshu,LI Xiaohui,YU Huimei,Guo Xinrong,ZHANG Dawei,CUI Jiarun. Study on the Application of Bullfrog Instead of Toad in Physiology Teaching Experiment[J]. Laboratory Animal Science, 2021, 38(6): 53-58
Authors:XU Caiyun  LI Tianshu  LI Xiaohui  YU Huimei  Guo Xinrong  ZHANG Dawei  CUI Jiarun
Abstract:Objective This study explored the feasibility of using bullfrogs to replace toads in physiology teachingexperiments by comparing the experimental effects between bullfrogs and toads in physiology teaching experiments.Method Bullfrogs and toads were separately used to carry out frog heart perfusion experiment, sciatic nervegastrocnemius muscle stimulation and contraction experiment.Result The frog heart perfusion experiment showed that the bullfrogs and toads under the action of Na+ , Ca2+ , K+ , norepinephrine, acetylcholine, lactic acid and sodium bicarbonate had the similiar effect on the activity of the isolated heart. In the sciatic nerve-gastrocnemius stimulating contraction experiment, the stimulation intensity was 0. 1 V caused minimal muscle contraction. Under the stimulation of around 0. 15 V, the muscle contraction amplitude reached maximum and remained urchanged. As the stimulation frequency increased, the skeletal muscles performed single contraction, incomplete tonic contraction and ionic contraction. After stimulated, the action potential conduction velocity of the nerve trunk about bulfrogs and toods is 16. 6 m / s and 13. 16 m / s respectively. There was no essential difference about experimetal results between the bullfrog group and the toad group ( P>0. 05) . Conclusion It is feasible solution that bullfrogs instead of toads to carry out physiology teaching experiments.
Keywords:bullfrog  toad,physiological experiment,
点击此处可从《实验动物科学》浏览原始摘要信息
点击此处可从《实验动物科学》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号