氢吗啡酮对炎性痛大鼠脊髓ERK1/2信号通路的影响

目的 探讨氢吗啡酮对炎性痛大鼠脊髓ERK1/2信号通路的影响.方法 选择SPF级雄性大鼠30只,随机分为3组,正常组、模型组和氢吗啡酮组;检测各组大鼠热痛阈和机械痛阈;ELISA检测血清中TNF-α、IL-6和IL-10的含量;Western blot检测各组大鼠脊髓中ERK1/2和p-ERK1/2蛋白表达水平;qRT...

Effect of Hydromorphine on ERK1 / 2 Signal Pathway in Spinal Cord of Rats with Inflammatory Pain

Abstract: Objective To investigate the effect of hydromorphine on ERK1 / 2 signal pathway in spinal cord of ratswith inflammatory pain. Method Thirty SPF male rats were randomly divided into three groups: the normalgroup, the model group and the hydromorphine group. Detection of thermal pain threshold and mechanical pain threshold were detected in rats of each group. TNF-α, IL-6 and IL-10 in serum were tested by ELISA. Western blot detected the ERK1 / 2 and p-ERK1 / 2 protein expression in spinal cord of rats in each group. The expression ofERK1 / 2 mRNA in spinal cord of rats in each group was detected by qRT-PCR method . Result Compared withthe normal group, the content of serum inflammatory cytokines TNF-α and IL-6 in the model group was significantly higher than that in the normal group, while the content of IL-10 in the model group was significantly lower than thatin the normal group, and the thermal pain threshold was significantly shortened and the mechanical pain domainwas significantly decreased in the model group ( P<0. 05) . The expression levels of ERK1 / 2 mRNA, ERK1 / 2 andp-ERK1 / 2 protein in spinal cord of the model group were significantly higher than those of the normal group ( P<0. 05) . After injection of hydromorphine, compared with the model group, the contents of serum TNF-α and IL-6 were significantly decreased, while the content of IL-10 was significantly increased, the thermal pain threshold wassignificantly prolonged and the mechanical pain domain was significantly increased ( P<0. 05) . The expression ofERK1 / 2 mRNA, ERK1 / 2 and p-ERK1 / 2 protein decreased significantly in hydromorphine group ( P < 0. 05 ) .Conclusion Hydromorphine can effectively improve complete freund ’ s adjuvant-induced inflammatory pain inrats. Its anti-inflammatory and analgesic mechanism is related to its reduction of the production of inflammatory factors and inhibition of the activation of ERK1 / 2 signal pathway in rats.