耐辐射奇球菌急性毒性研究

目的 探讨耐辐射奇球菌(Deinococcus radiodurans,DR)活菌及细菌破碎物的急性毒性.方法 分别将DR高浓度1×109/mL(高浓度活菌组)、低浓度1×107/mL(低浓度活菌组)、细菌破碎物高浓度1×109/mL(高浓度细菌破碎物组)、低浓度1×107/mL(低浓度细菌破碎物组)以0.4 mL/1...

Study on Acute Toxicity of Deinococcus radiodurans

Abstract: Objective To investigate the acute toxicity of Deinococcus radiodurans ( DR) and its broken products.Method The KM mice were administrated with 0. 4 mL / 10 g of 1×10⁹ / mL DR ( high concentration DR group) , 1×10⁷ / mL DR ( low concentration DR group) , 1 × 10⁹ / mL DR broken products ( high concentration DR debris group) , 1×10⁷ / mL DR broken products ( low concentration DR debris group) and Tryptone Glucose Yeast agarmedium ( TGY medium group) , respectively. After intragastrically administrated twice a day every 8 hours, the mice were observed for 14 days, then the organ coefficient, body weight, blood routine ( white blood cell, lymphocyte, red blood cell, hemoglobin, hematocrit, mean corpuscular volume, platelet) and plasma biochemical indexes ( alanine aminotransferase, aspartate aminotransferase, total protein, creatinine, creatine kinase, magnesium, phosphorus, creatine kinase MB isoenzyme, lactate dehydrogenase) were used to analyze and evaluatethe toxicity of DR. Result No abnormal secretion, defecation, respiration and movement were found in mice indifferent dose groups. From 0. 5 hour after administration to the end of the experiment, no abnormalities such as slow reaction, pupil change, eyeball protrusion, eyelid ptosis and skin color change were observed in the test mice.There were no significant differences in body weight, blood routine test and blood biochemical indexes in the trialgroups compared with TGY group. The spleen coefficient was significantly increased in the low-dose group, and thespleen pathological examination showed that there was no substantial lesion. Conclusion The live and broken DRhave no acute toxicity to mice. This experiment provides experimental basis for clinical application of DR.