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Differential display of carrot radicle genes in different developmental states
Authors:Chunxia Chen  Meijuan Huang  Hao Liu  Naihu Wu
Institution:(1) Institute of Developmental Biology, Chinese Academy of Sciences, 100080 Beijing;(2) School of Life Sciences, Peking University, 100871 Beijing
Abstract:mRNA differential display was used in analyzing the expression of carrot somatic embryo radicle gene in different developmental stages brought about by controlled cultivation. As shown by experiment, marked differences in electrophoresis patterns of DNA exist between the normally developing carrot radicle and those with their development inhibited. 12 series of specific bands have been cloned, and structural analysis of them is under way.
Keywords:carrot  development and differentiation of root  mRNA differential display  gene expression
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