高效液相色谱法同时测定海产品中四种抗生素的分析方法

建立了同时检测海产品中土霉素、四环素、金霉素、氯霉素残留的高效液相色谱分析方法，以醋酸铵-醋酸/水缓冲液和乙腈作为流动相，通过二元梯度洗脱，使四种抗生素获得了较好的分离，以基线噪音的3倍计算，土霉素、四环素、金霉素、氯霉素最低检出浓度分别为32μg/kg、15μg/kg、23μg/kg、19μg/kg，在添加浓度范围内，四种抗生素的加标回收率为76~92%，相对标准偏差为0.8~4.5%，该方法快速、灵敏、准确，可靠性高，可用于海产品中抗生素残留的色谱分析。

Simultaneous determination by HPLC of four kinds of antibiotics in seafood

A method for simultaneous determination of oxytetracycline, tetracycline, chlortetracycline and choramphenicol in seafood was successfully developed using high performance liquid chromatography with ammonium acetate/water and acetonitrile as the mobile phase. The results showed that the four antibiotics were well separated under the optimum conditions, with the limits of detection being 32 μg/kg for oxytetracycline, 15 μg/kg for tetracycline, 23 μg/kg for chlortetracycline, and 19 μg/kg for choramphenicol. The recovery of standard additions was 76~92% and the relative standard deviation was 0.8-4.5%. The method is rapid, sensitive, accurate and reliable and can be applied in the analysis of antibiotic residues in seafood.