| 邻菲罗啉衍生物与人端粒G-四链体DNA的相互作用及细胞活性研究 |
| |
| 引用本文： | 王立华,文烨,刘洁,张芳芳,魏春英.邻菲罗啉衍生物与人端粒G-四链体DNA的相互作用及细胞活性研究[J].山西大学学报(自然科学版),2012(3):520-524. |
| |
| 作者姓名： | 王立华 文烨 刘洁 张芳芳 魏春英 |
| |
| 作者单位： | 山西大学分子科学研究所 |
| |
| 基金项目： | 国家自然科学基金(21171108);教育部博士点专项基金(20101401110009);山西省自然科学基金(2009011012-2);太原市大学生创新创业项目(20103007) |
| |
| 摘 要： | 通过紫外可见吸收滴定,荧光滴定和聚合酶链反应停止分析(PCR stop assay)方法研究了3个邻菲罗啉衍生物1-3与人端粒G-四链体DNA在包含100mmol·L-1 KCl的磷酸缓冲体系中的相互作用,并用MTT法研究了化合物对人肝癌细胞HepG2增殖的影响.结果表明:这三个化合物都能与G-四链体相互作用并显著稳定其结构,结合常数约为1×106 mol-1·L;同时这些化合物能在微摩尔浓度抑制HepG2细胞增殖.
|
| 关 键 词： | 邻菲罗啉 端粒 G-四链体 细胞增殖 |
Studies on the Interactions of Phenanthroline Derivatives with Human Telomere G-quadruplex DNA and on Their Effects on the Cell Activity |
| |
| WANG Li-hua,WEN Ye,LIU Jie,ZHANG Fang-fang,WEI Chun-ying.Studies on the Interactions of Phenanthroline Derivatives with Human Telomere G-quadruplex DNA and on Their Effects on the Cell Activity[J].Journal of Shanxi University (Natural Science Edition),2012(3):520-524. |
| |
| Authors: | WANG Li-hua WEN Ye LIU Jie ZHANG Fang-fang WEI Chun-ying |
| |
| Institution: | (Institute of Molecular Science,Shanxi University,Taiyuan 030006,China) |
| |
| Abstract: | The interactions between three Phenanthroline derivatives and the human telomeric G-quadruplex DNA were studied by absorption titration,fluorescence titration and PCR stop assay in PBS solution containing 100 mmol·L-1 KCl.In addition,MTT assay was used to study the effect of the compounds on the proliferative activity of human HepG2 cells.The results showed that the compounds could bind G-quadruplex with affinity constant of 1×106 mol-1·L,and stabilized significantly the structure of G-quadruplex.At the same time,the compounds could inhibit the proliferation of HepG2 cells in micromolar concentration. |
| |
| Keywords: | Phenanthroline telomere G-quadruplex cell proliferation |
| 本文献已被 CNKI 等数据库收录！ |
