| 鼠白细胞介素15真核表达质粒的构建及活性鉴定 |
| |
| 引用本文： | 姜春来,马红霞,于湘晖,吴永革,李惟,孔维.鼠白细胞介素15真核表达质粒的构建及活性鉴定[J].吉林大学学报(理学版),2005,43(2):238-240. |
| |
| 作者姓名： | 姜春来 马红霞 于湘晖 吴永革 李惟 孔维 |
| |
| 作者单位： | 吉林大学生命科学学院疫苗研究中心， 长春 130023 |
| |
| 基金项目： | 国家自然科学基金(批准号:30371317) |
| |
| 摘 要： | 从经PMA刺激的小鼠脾细胞中提取总RNA， 采用RT-PCR技术获得鼠白细胞介素15(mIL-15)基因， 将其克隆至真核表达质粒VR1012中， 转染COS-7细胞， 将转染细胞裂解， Western blot检测. 表明获得了mIL-15真核表达载体， 其具有完整的带有信号肽的mIL-15序列， 重组结果正确， Western blot检测阳性. 并获得有生物活性的mIL-15蛋白.
|
| 关 键 词： | 鼠白细胞介素15 克隆 活性鉴定 |
| 文章编号： | 1671-5489(2005)02-0238-03 |
| 收稿时间： | 2004-08-05 |
| 修稿时间： | 2004年8月5日 |
Cloning and Expression of Recombinant mIL-15 and Bioactivity Assay |
| |
| JIANG Chun-lai,MA Hong-xia,YU Xiang-hui,WU Yong-ge,LI Wei,KONG Wei.Cloning and Expression of Recombinant mIL-15 and Bioactivity Assay[J].Journal of Jilin University: Sci Ed,2005,43(2):238-240. |
| |
| Authors: | JIANG Chun-lai MA Hong-xia YU Xiang-hui WU Yong-ge LI Wei KONG Wei |
| |
| Institution: | Vaccine Research Center, College of Life Science, Jilin University, Changchun 130023, China |
| |
| Abstract: | Murine splenocyte was harvested after the mouse was stimulated by PMA, the cells were extracted for total RNA and RT-PCR, and IL-15 eukaryotic express plasmid was then constructed. COS-7 cell line was transfected by this plasmid, supernatant and the cells were harvested. The supernatant was serially by diluted to detect the bioactivity and express level by western blot. This recombinant murine IL-15 eukaryotic plasmid can increase immune response as DNA vaccine adjuvant potentially. |
| |
| Keywords: | mIL-15 clone biological activity assay |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《吉林大学学报(理学版)》浏览原始摘要信息 |
| 点击此处可从《吉林大学学报(理学版)》下载免费的PDF全文 |
