中国少数民族全基因组DNA高通量提取及其样本库建立

为保存中国不同民族基因组遗传资源,建立中国少数民族全基因组DNA样本库.按照临床采血标准,遵守知情同意原则,采集不同民族志愿者外周血2 mL,采用全自动核酸提取设备高通量提取DNA,经质量控制后编号入库,建立了包含545例样本的中国少数民族全基因组DNA样本库.本研究将为研究人类起源、环境与遗传背景相互作用提供物质基础,为探索疾病病因、诊断技术和治疗策略提供资源保障.

High-throughput extraction and establishment of sample bank of the genome-wide DNA of different Chinese ethnic minorities

To preserve genetic resources of different Chinese ethnic groups’ genomes and establish the sample bank of the genome-wide DNA,2 mL peripheral blood was collected from each volunteer of different ethnic minorities according to the clinical blood collection standards and complying with the principle of informed consent.Automatic nucleic acid extraction machine was used to conduct high-throughput extraction of all blood samples,and the samples were numbered and put in the bank after passing quality control.The established sample bank of the genome-wide DNA includes 545 samples from ethnic minorities.The bank will provide material basis for the research of anthropogenesis and interaction of environment and genetic background;it will also serve as a resource for disease treatment and diagnostics.