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头孢曲松钠与胃蛋白酶相互作用的光谱研究
引用本文:肖敏,邵从英,葛宝宝,訾言勤. 头孢曲松钠与胃蛋白酶相互作用的光谱研究[J]. 淮北煤炭师范学院学报(自然科学版), 2014, 0(4): 27-31
作者姓名:肖敏  邵从英  葛宝宝  訾言勤
作者单位:淮北师范大学 化学与材料科学学院,安徽 淮北,235000
基金项目:安徽省教育厅自然科学基金项目
摘    要:在不同温度下利用荧光光谱、紫外吸收光谱等方法研究胃蛋白酶与头孢曲松钠之间的相互作用.计算两者的结合常数K和结合位点数n,判断头孢曲松钠对胃蛋白酶荧光猝灭机制为动态猝灭.由热力学参数ΔG<0,ΔH>0且ΔS>0,表明结合过程是自发进行且两者之间的作用力主要是疏水作用力.通过F?rster偶极-偶极非辐射能量转移机理确定头孢曲松钠在胃蛋白酶中与色氨酸残基之间距离R为0.92 nm.

关 键 词:头孢曲松钠  胃蛋白酶  荧光猝灭  紫外光谱  相互作用

Spectroscopic Study on the Interaction Between Ceftriaxone Sodium and pepsin
XIAO Min,SHAO Cong-ying,GE Bao-bao,ZI Yan-qin. Spectroscopic Study on the Interaction Between Ceftriaxone Sodium and pepsin[J]. Journal of Huaibei Coal Industry Teachers College(Natural Science edition), 2014, 0(4): 27-31
Authors:XIAO Min  SHAO Cong-ying  GE Bao-bao  ZI Yan-qin
Affiliation:( School of Chemistry and Materials Science, Huaibei Normal University, 235000,Huaibei, Anhui, China )
Abstract:The interaction between ceftriaxone sodium and pepsin was investigated by spectrophotometric techniques such as fluorescence and UV-vis absorption at different temperatures.The binding constant K and the number of binding site n of the system were calculated.The results showed that ceftriaxone sodium has a strong quenching on the intrinsic fluorescence of pepsin through a dynamic quenching procedure.The nega?tive value of G0 reveals that the binding process is a spontaneous process and the positive value of ΔH/ΔS in?dicates that the binding power between them is mainly hydrophobic bond.It was determined that the distance between ceftriaxone sodium and tryptophan residues in pepsin is 0.92 nm by the mechanism of non-radiation energy transfer.
Keywords:ceftriaxone sodium  pepsin  fluorescence quenching  UV-vis absorption  interaction
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