葡甘聚糖-壳聚糖复合膜的制备及细胞相容性评价

采用溶液共混法制备了葡甘聚糖-壳聚糖复合膜,并测定了材料的吸水率等理化性能。模拟体内生理条件用溶菌酶对复合膜进行体外酶解实验,并与壳聚糖膜作了比较;以体外培养的小鼠成纤维细胞(N IH 3T 3)为对象,利用复合膜的浸渍液培养与膜材料表面直接培养法,同时结合复合膜的溶血反应实验,研究了复合膜的细胞相容性。结果表明:溶菌酶对复合膜的生物降解有促进作用,复合膜降解速率较壳聚糖膜快。四甲基偶氮唑盐比色法(MTT法)实验表明复合膜浸渍液对细胞无毒性效应,显微镜和扫描电镜显示N IH 3T 3在复合膜上能很好地贴附,生长旺盛。复合膜溶血率小于5%。葡甘聚糖-壳聚糖复合膜具有良好的生物降解性和细胞相容性。

Fabrication of Konjac Glucomannan-Chitosan Blend Films and Its Cytocompatible Evaluation

Konjac glucomannan-chitosan blend films were prepared by solution method.The performance of the films such as the water absorption were investigated.The cell compatibility of the blend films was studied by both hemolytic reaction and cultivating fibroblasts(NIH3T3) in blend film steeping fluid or on the surface of the blend film in vitro.Enzymolysis imitating in vivo natural condition of the konjac glucomannan-chitosan blend films were compared with that of chitosan film.Lysozyme could promote biodegradation of the blend film,and the blend films were biodegraded faster than chitosan films.MTT colorimetric assay results indicate that the blend film steeping fluid has no toxic effect to cell.Fibroblasts NIH3T3 observed with microscope and scanning electron microscope can be attached to the blend film well and grew vigorously.The hemolysis rate of the blend film is less than 5%.Results suggest that the konjac glucomannan-chitosan blend film has the properties of good biodegradability and cell compatibility.