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| 黄曲霉素M1间接竞争ELISA的建立及其在牛奶中的应用 | |
| 引用本文： | 陈曦,孟萌,Sergei A Eremin,许迎辉,尹永梅,郗日沫.黄曲霉素M1间接竞争ELISA的建立及其在牛奶中的应用[J].科学技术与工程,2013,13(19):5455-5458. |
| 作者姓名： | 陈曦 孟萌 Sergei A Eremin 许迎辉 尹永梅 郗日沫 |
| 作者单位： | 1. 天津科技大学生物工程学院,工业发酵微生物教育部重点实验室,天津市工业微生物重点实验室,天津300457 2. 南开大学药学院,南开大学药物化学生物学国家重点实验室,天津市分子药物研究重点实验室,天津300071 3. 莫斯科大学化学学院,莫斯科,119991 4. 河北省石家庄市餐饮化保执法大队,石家庄,050000 |
| 基金项目： | 天津市自然科学基金（No. 11ZCGHHZ01200；No. 12JCQNJC08900）；中央高校基本科研业务费（65011751） |
| 摘 要： | 利用高特异性单克隆抗体,建立黄曲霉素M1的间接竞争ELISA检测方法。棋盘法优化了包被抗原及酶标抗体的最佳稀释倍数。包被抗原和酶标抗体的最佳工作浓度分别为1∶15000和1∶2000。该方法的线性范围为(0.1—8.1)ng·mL-1。除了与黄曲霉素B1的交叉反应为35%外,与黄曲霉素B2、黄曲霉素G1及黄曲霉素G2的交叉反应均小于1%。在牛奶实际样品检测的回收率均大于70%,而且检测过程在10min以内即可完成。该方法灵敏度高,特异性强,而且操作简单,适合多样本的快速筛查,为黄曲霉素M1的高效检测提供了一个良好的选择。 |
| 关 键 词： | 黄曲霉素M1 间接竞争ELISA 交叉反应 牛奶 |
| 收稿时间： | 2013/3/21 0:00:00 |
| 修稿时间： | 2013/3/21 0:00:00 |
Development of an Indirect Competitive ELISA for Rapid Analysis of Aflatoxin M1 in Milk |
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| CHEN Xi , MENG Meng , Sergei A Eremin , XU Ying-hui , YIN Yong-mei , XI Ri-mo.Development of an Indirect Competitive ELISA for Rapid Analysis of Aflatoxin M1 in Milk[J].Science Technology and Engineering,2013,13(19):5455-5458. | |
| Authors: | CHEN Xi MENG Meng Sergei A Eremin XU Ying-hui YIN Yong-mei XI Ri-mo |
| Institution: | 2* ( Key Laboratory of Ministry of Education Industrial Fermentation Microbiology,Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology,Tianjin University of Science &Technology1 ,Tianjin 300457,P. R. China; State Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Molecular Drug Research2 , College of Pharmacy,Nankai University,Tianjin 300071,P. R. China; Faculty of Chemistry,M. V. Lomonosov Moscow State University3 ,Moscow 119991,Russia; Law Enforcement Unit for Cosmetics4 ,Health Products and Food,Shijiazhuang Food and Drug Administration,Shijiazhuang 050000,P. R. China) |
| Abstract: | An indirect competitive ELISA for Aflatoxin M1 was developed based on anti-AFM1 monoclonal antibody. Firstly, the working concentration of coated antigen and enzyme labeled antibody was optimized and their dilution ratios were selected as 1:15000 and 1:2000 respectively. The linear range of the proposed ELISA was in 0.1-8.1 ng mL-1. The cross-reactivities to AFB2, AFG1 and AFG2 were all below 1%, while that of AFB1 was 35%. The recoveries in milk samples were all higher than 70% and the detection could be finished within 10 min. It can be concluded that this method for AFM1 analysis was sensitive, specific, simple and rapid which exhibited a potentiality of screening a large number of samples on site. It also provided an option for effective determination of AFM1. |
| Keywords: | Aflatoxin M1 indirect competitive ELISA cross-reactivity milk |
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