Construction of a site-specific, deletion-frameshift mutation in an essential gene of bacteriophage phiX174. |
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Authors: | M Z Humayun R W Chambers |
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Abstract: | By in vitro methods, we have deleted 80 nucleotides from a preselected site in the coding sequence of gene G of bacteriophage phiX174. This mutant (Gdelta/FS ZH1) also carries a--2 frameshift. The mutation is lethal, but mutant virus can be stably maintained on host strains bearing plasmids carrying phiX gene G. |
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