Inhibiting mechanism of baculovirus p35 gene to apoptosis |
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Authors: | Li Xiaofeng Qi Yipeng Lin Hong Li Yan |
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Institution: | (1) College of Life Sciences, Wuhan University, 430072 Wuhan, China |
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Abstract: | We transfected Sf9 cells with an expressing vector p35IE1Neo containing antiapoptotic p35 gene and neomycin-resistant gene
(as a selection marker). By G418 screening, we got transformed cells that appeared resistant to G418 and picked one clone
named Sf9-35. By hybridization in situ, it was found that p35 gene had integrated into the chromosome of Sf9-35 cells; By
using actinomycin D treatment and cellular DNA electrophoresis, Sf9-35 cells were found to resist apoptosis induced by infection
of vAcΔp35 deleting p35 gene and actinomycin D treatment; And it was also found that apoptosis induced by viral infection
and actinomycin D treatment can only be delayed, but can not be stopped in Sf9-35.
Supported by the National Natural Science Fundation of China
Li Xiaofeng: born in 1965, Lecturer |
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Keywords: | apoptosis transformed cells p35 gene baculovirus |
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