| 水稻悬浮细胞系的建立 |
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| 引用本文： | 袁力勇,李绍清,李阳生,冯双华,戴长柏,李达模.水稻悬浮细胞系的建立[J].云南大学学报(自然科学版),2003,25(4):373-376. |
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| 作者姓名： | 袁力勇 李绍清 李阳生 冯双华 戴长柏 李达模 |
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| 作者单位： | 1. 中国医学科学院,协和医科大学,医学生物学研究所,云南,昆明,650118
2. 中国科学院,中国科技大学,长沙农业现代化研究所,湖南,长沙,410125 |
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| 摘 要： | 对包括广亲和材料、恢复系、花培后代在内的30个水稻不同基因型的品种(系)在MS培养基中的培养能力进行了调查.根据出愈率的高低和愈伤组织培养能力,把它们分成Ⅰ,Ⅱ,Ⅲ3种类型.从Ⅰ类型中挑选CPSLO17和IR21820-154-3-2-2-8用N6液体培养基进行液体培养,CPSLO17建立了悬浮细胞系,证实基因型的差异是决定建立良好悬浮细胞系的关键因素之一.此外还考察了低锌胁迫对水稻悬浮细胞系和幼胚愈伤分化率的影响.
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| 关 键 词： | 水稻 悬浮细胞系 基因型 低锌胁迫 |
| 文章编号： | 0258-7971(2003)04-0373-04 |
| 修稿时间： | 2002年11月20 |
The establishment of cell suspension culture of rice |
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| YUAN Li-yong,LI Shao-qing,LI Yang-sheng,FENG Shuang-hua,DAI Chang-bo,LI Da-mo.The establishment of cell suspension culture of rice[J].Journal of Yunnan University(Natural Sciences),2003,25(4):373-376. |
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| Authors: | YUAN Li-yong LI Shao-qing LI Yang-sheng FENG Shuang-hua DAI Chang-bo LI Da-mo |
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| Institution: | 1. Institute of Medical Bioligy, Chinese Accdemy of Medical Sciences/Peking Union Medical College, Kunming 650118, China;2. Changsha Institute of Argricultural Modernization, The Chinese Academy of SciencesUniversity of Science Technology of China, Changsha 410125, China |
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| Abstract: | Immature and mature embryos of 30 rice varieties(lines)were selected and cultured on MS solid medium.According to the callus induction ratio and their response to subculture,they were classified into three groups.CPSLO17 and IR21820-3-2-2-8 showed high callus induction ratio and good response to subculture,were selected to establish the cell suspension culture with N6 liquid medium.The results showed that the genotype is one of the key factors to establish the cell suspensions.With zinc deficiency,the P,Zn contents in suspension cell lines were lower,while P/Zn,Fe/Zn values higher,which is just as the change of mineral elements in rice plant under zinc deficiency.On the other hand,there was little effect on the callus induction frequency,but the differentation ratio decreased under zinc deficiency. |
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| Keywords: | rice suspension cells genotypes zinc deficiency |
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