| Medium Optimization of Tetraselmis sp.—1 Using Response Surface Methodology |
| |
| 引用本文： | 沈继红,Yu Junhong,Lin Xuezheng,Li Guangyou,Liu Fayi.Medium Optimization of Tetraselmis sp.—1 Using Response Surface Methodology[J].高技术通讯(英文版),2003,9(2):27-30. |
| |
| 作者姓名： | 沈继红 Yu Junhong Lin Xuezheng Li Guangyou Liu Fayi |
| |
| 作者单位： | FirstInstituteofOceanographySOA,Qingdao266061,P.R.China |
| |
| 摘 要： | Response surface methodology (RSM) is used to optimize the medium of Tetraselmis sp.-1 which is cell fused microalgae capable of growing under mixotrophie condition. Empirical models are developed to describe the relationships between the operating variables (glucose, urea, sodium dehydrogenate phosphate, sodium chloride) and responses (cell density). Statistical analysis indi-cates that glucose and urea have significant effects on the microalgae cell density, but other two factors (sodium dehydrogenate phosphate, sodium chloride) have no obvious effect. The path of steepest ascent is used to approach the optimal region of medium ecanposition. Optimal cell density(2. 638 g dry weight/L) was reached when the operating conditions were glucose concentration(30.75 % ), urea concentration (0.440 g/L), sodium dehydrogenate phosphate (15mg/L) and sodium chloride (28 g/L).
|
| 关 键 词： | 微藻 细胞融合 表面响应方法 媒质 最优化 氯化钠 磷酸钠 |
Medium Optimization of Tetraselmis sp.-1 Using Response Surface Methodology |
| |
| Yu Junhong,Lin Xuezheng,Li Guangyou,Liu Fayi.Medium Optimization of Tetraselmis sp.-1 Using Response Surface Methodology[J].High Technology Letters,2003,9(2):27-30. |
| |
| Authors: | Yu Junhong Lin Xuezheng Li Guangyou Liu Fayi |
| |
| Abstract: | Response surface methodology (RSM) is used to optimize the medium of Tetraselmis sp.-1 which is cell fused microalgae capable of growing under mixotrophic condition. Empirical models are developed to describe the relationships between the operating variables (glucose, urea, sodium dehydrogenate phosphate, sodium chloride) and responses (cell density). Statistical analysis indicates that glucose and urea have significant effects on the microalgae cell density, but other two factors (sodium dehydrogenate phosphate, sodium chloride) have no obvious effect. The path of steepest ascent is used to approach the optimal region of medium composition. Optimal cell density (2.638 g dry weight/L) was reached when the operating conditions were glucose concentration (30.75 %), urea concentration (0.440 g/L), sodium dehydrogenate phosphate (15 mg/L) and sodium chloride (28 g/L). |
| |
| Keywords: | response surface methodology (RSM) cell fused microalgae medium optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
