| 浓香型大曲微生物群落结构的PCR-SSCP分析条件优化 |
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| 引用本文： | 罗惠波,李浩,黄治国,张强,卫春会,沈才萍.浓香型大曲微生物群落结构的PCR-SSCP分析条件优化[J].四川理工学院学报(自然科学版),2009,22(4):72-74. |
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| 作者姓名： | 罗惠波 李浩 黄治国 张强 卫春会 沈才萍 |
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| 作者单位： | 四川理工学院酿酒生物技术及应用四川省重点实验室,四川,自贡,643000 |
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| 基金项目： | 四川省科技支撑项目，四川省应用基础项目，泸州老窖股份有限公司科研奖学金资助 |
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| 摘 要： | 文章研究了浓香型大曲微生物群落结构的PCR-SSCP分析条件的优化,结果表明:PCR产物100℃变性5 min后,在交联度为49:1,浓度为8%的聚丙烯酰胺凝胶中,无甘油,4℃条件下200 V电泳15 h,可得到较理想的图谱。
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| 关 键 词： | PCR-SSCP 条件优化 微生物群落 |
Optimal Conditions of PCR-SSCP Technology for the Analysis of Microbial Communities in Luzhou-flavor Daqu |
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| LUO Hui-bo,LI Hao,HUANG Zhi-guo,ZHANG Qiang,WEI Chun-hui,SHEN Cai-ping.Optimal Conditions of PCR-SSCP Technology for the Analysis of Microbial Communities in Luzhou-flavor Daqu[J].Journal of Sichuan University of Science & Engineering:Natural Science Editton,2009,22(4):72-74. |
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| Authors: | LUO Hui-bo LI Hao HUANG Zhi-guo ZHANG Qiang WEI Chun-hui SHEN Cai-ping |
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| Institution: | Liquor-making Biotechnology & Application key Laboratory of Sichuan Province;Sichuan University of Science & Engineering;Zigong 643000;China |
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| Abstract: | The optimal conditions of PCR-SSCP Technology for the analysis of microbial communities in Luzhou-flavor Daqu is studied in the paper.The results show that under the condition of the concentration of the gel being 8%,the proportion of acrylamide and N,N'-methylene-bisacrylamidei being 49:1,without glycerine,denaturing at 100℃for 5 min,4℃, 200 V,electrophoresis 15 h,perfect spectrogram can be achieved. |
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| Keywords: | PCR-SSCP |
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