| 高产碱性蛋白酶工程菌的构建及鉴定 |
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| 引用本文： | 潘延云,周艳芬,张贺迎,吴宝东. 高产碱性蛋白酶工程菌的构建及鉴定[J]. 哈尔滨师范大学自然科学学报, 2003, 19(4): 97-100 |
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| 作者姓名： | 潘延云 周艳芬 张贺迎 吴宝东 |
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| 作者单位： | 1. 河北大学;河北师范大学
2. 河北大学 |
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| 基金项目： | 河北省自然科学基金资助项目 |
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| 摘 要： | 利用原生质体融合技术，使碱性蛋白酶生产菌2709与枯草杆菌BD105(含有碱性蛋白酶基因克隆载体pDW2)杂交，得到1株高产碱性蛋白酶的工程菌A16．A16的表型与生长特征与2709相似，相同条件下发酵酶活性比2709菌株高50％，摇瓶发酵的产酶水平最高可达30000u／mL。用菌落的原位杂交鉴定出该菌株携有双亲的遗传物质．
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| 关 键 词： | 工程菌 原生质体融合 碱性蛋白酶 地衣芽孢杆菌2709 发酵酶 |
THE CONSTRUCTION AND GENETICS IDENTIFICATION OF ENGINEERING STRAIN PRODUCING ALKALINE PROTEASE |
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| Pan Yanyun Zhou Yanfen Zhang Heying Wu Baodong. THE CONSTRUCTION AND GENETICS IDENTIFICATION OF ENGINEERING STRAIN PRODUCING ALKALINE PROTEASE[J]. Natural Science Journal of Harbin Normal University, 2003, 19(4): 97-100 |
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| Authors: | Pan Yanyun Zhou Yanfen Zhang Heying Wu Baodong |
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| Abstract: | A engineering strain with a high ability of producing alkaline protease was obtained by fusing protoplasts of Bacillus licheniformis 2709 and Bacillus subtilis BD 105 [pDW 2(pMK 4 and alkaline protease gene)]. Cell proliferation aspect and phenotype of A16 was similar to that of 2709, and its activities of fermentation were 50%, higher that that of 2709 under the same conditions. The maximum activities of protease were 30000 u/mL from A16 in the optimum fermenntation. In situ hybridization data indicated that A16 carried genes from BD105 (pDW 2) and B. licheniformis 2709. |
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| Keywords: | Alkaline protease Protoplast fusion Bacillus licheniformis 2709 |
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