| 靛蓝二磺酸钠与脱氧核酸的相互作用研究 |
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| 引用本文： | 王峰,黄薇,刘涛. 靛蓝二磺酸钠与脱氧核酸的相互作用研究[J]. 枣庄师专学报, 2008, 25(2): 45-47 |
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| 作者姓名： | 王峰 黄薇 刘涛 |
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| 作者单位： | 枣庄学院化学化工系,山东枣庄277160 |
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| 摘 要： | 本文采用紫外光谱、荧光光谱等手段对靛蓝二磺酸钠与小牛胸腺脱氧核糖核酸（ctDNA）的作用方式进行了研究，发现核糖对靛蓝二磺酸有较强的荧光猝灭作用，并测定了其反应的结合常数，结合位点数和基本热力学参数等，紫外吸收光谱证实了靛蓝二磺酸钠通过嵌插作用与核酸结合．
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| 关 键 词： | 荧光光谱 靛蓝二磺酸钠 小牛胸腺脱氧核糖核酸 |
| 文章编号： | 1004-7077(2008)02-0045-03 |
| 修稿时间： | 2007-12-20 |
Spectralmetric Studies on the Interaction between Indigotine Disulfonate and Deoxyribonucleic Acid |
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| WANG Feng,HUANG Wei,LIU Tao. Spectralmetric Studies on the Interaction between Indigotine Disulfonate and Deoxyribonucleic Acid[J]. Journal of Zaozhuang Teachers' College, 2008, 25(2): 45-47 |
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| Authors: | WANG Feng HUANG Wei LIU Tao |
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| Affiliation: | (Department of Chemistry, Zaozhuang University, Zaozhuang 277160,China) |
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| Abstract: | The characteristics of the interaction between indigotine disulfonate （ID） and calf thymus deoxyribonucleic acid （ctDNA） were studied by using UV -Vis spectra and fluorescent spectra. It is shown that ID has a powerful ability to quench the fluorescence intensity of ctDNA. The results show that ID interacts with ctDNA in the mode of intercalation. |
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| Keywords: | Fluorescence spectrum Indigotine disulfonate Calf thymus deoxyribonucleic acid |
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