活性污泥性质对基因工程菌吸附影响研究

基因工程菌在活性污泥中的生存状况是决定其生物强化作用的关键因素,活性污泥吸附对基因工程菌生存状况具有重要影响。在典型活性污泥中,考察了吸附于活性污泥的基因工程菌生存状况,以及活性污泥性质对其基因工程菌吸附能力的影响。结果表明,基因工程菌吸附于污泥絮体后,更有利于其生存。污泥质量浓度增大,吸附能力减小;污泥粒径减小,吸附能力增加;污泥EPS含量越高,吸附能力越强。同时,在相同污泥质量浓度下,普通活性污泥吸附能力大于MBR污泥,表明污泥有机质含量比污泥粒径对基因工程菌吸附的影响更显著。在接种密度为105～1014CFU/mL时,普通活性污泥和MBR污泥对基因工程菌的吸附基本符合Freundlich等温吸附方程。

Influence of activated sludge properties on adsorption of genetically engineered microorganism (GEM)

Living status of genetically engineered microorganism(GEM) in the activated sludge is the key factor influencing bioaugmented treatment effect.The activated sludge adsorption is very important to its survival.The survival of GEM after inoculated into typical activated sludge is investigated and the effects of activated sludge properties on adsorption of GEM are also explored.The result show that adsorption by sludge flocs is more helpful for GEM survival after inoculation.When sludge concentration increases,adsorption capacity of GEM by activated sludge decreases.As floc size decreases,adsorption capacity of GEM increases.In addition,adsorption capacity of GEM increases with an increase in sludge EPS content.The adsorption capacity of normal activated sludge is higher than that of MBR sludge at the same sludge concentration,indicating that the organic content of activated sludge has more influence than floc size on adsorption of GEM.The adsorption of GEM by both normal activated sludge and MBR sludge could be fitted by Freundlich adsorption equation when the inoculating GEM density varied from 105 CFU/mL to 1014 CFU/mL.