一种有固氮能力的节杆菌菌株的分离和初步鉴定

从日本冲绳县土壤采样经垃圾处理场堆肥化处理，分离到一株有固氮能力的菌株HS-G8，该菌为革兰氏阳性菌，不产芽孢，无运动性．在LB固体培养基上，菌落为黄色，圆形，边缘整齐，有光泽．有明显的杆-球状的生长周期，(1d内呈杆状，2d以上呈球状)．对该菌株HS-G8的16S rDNA的全序列测序并进行DNA同源性分析，发现它与Arthrobacter属的关系最近，与Arthrobacter nifotianae的同源性为99．1％．其主要生理生化特征也与Arthrobacter属相符．初步鉴定HS-G8可能为Arthrobacter属的一个新种。

Isolation and primary identification of a new nitrogng-fixation Arthrobacter strain

A bacterial strain HS-G_8 was isolated from the garbage heap in Japan, gram-positive, non-endospore, non-motile, azotobacter. The colonies on LB plate tended to appear yellow-pigment glossy circulars. Obvious growth period of bacilliform-micrococcus was observed. The full-length of 16S rDNA sequence of strain HS-G_8 was determined and analyzed. Strain HS-G_8 was most related to the genus of Arthrobacter. The homologous of nucleartides between HS-G_8 and Arthrobacter nicotianae was 99.1% and the main biochemical and physiological features of strain HS-G_8 were very similar to that of Arthrobacter. Since nitrogen-fixtion has not been observed previously among Arthrobacter sp., strain HS-G_8 was identified as a new Arthrobacter species which hold the ability of nitrogen-fixtion.