| 水稻同源重组型质粒载体的构建 |
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| 引用本文： | 徐虹,章军,楼士林,杨汉金. 水稻同源重组型质粒载体的构建[J]. 厦门大学学报(自然科学版), 1999, 38(6): 924-930 |
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| 作者姓名： | 徐虹 章军 楼士林 杨汉金 |
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| 作者单位： | 厦门大学生物学系,厦门,361005 |
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| 摘 要： | 根据同源重组机制,以水稻17S-5.8SrDNA 2.5 kb 同源片段和含有MT基因、Km r 基因的外源DNA片段构建了质粒载体pURKMT,并用电激法转化水稻愈伤组织,经Km 筛选,获得了对Km 抗性明显高于基础抗性的愈伤组织.经DNA斑点杂交检测,证实外源基因已整合到水稻基因组DNA中.
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| 关 键 词： | 水稻 质粒载体 电激转化 |
A New Trangenic Plasmid Vector For Oryza Sativa L. |
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| Xu Hong,Zhang Jun,Lou Shilin,Yang Hanjin. A New Trangenic Plasmid Vector For Oryza Sativa L.[J]. Journal of Xiamen University(Natural Science), 1999, 38(6): 924-930 |
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| Authors: | Xu Hong Zhang Jun Lou Shilin Yang Hanjin |
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| Abstract: | According to them echanism ofhom ologous recom bination, a new plasm id vector pURKMT was constructed, w hich contained Kanam ycin resistance gene, Metalloth- ionein geneand 2.5 kb hom ologous fragm entofOryza Sativa L. including 17S-5.8Sribosom al DNA. Then, donor plasm id pURKMT was introduced into intactrice calliby electrporation. By selection by kanam ycin, the transform antcalli, w hose kanam ycin resistance w as obviously higher than basic resistance, w ere obtained. Dot blotting data show ed that the foreign DNA had been integrated into rice genom e DNA. The results provided a new plasm id vector for transform ation offoreign gene in Oryza Sativa L.. |
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| Keywords: | Oryra sativa L. Plasm id vector Electroporation |
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