| DMSO诱导烟草BY-2悬浮细胞的凋亡 |
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| 引用本文： | 韩利波,梁蒙,李佳雯,陈志玲.DMSO诱导烟草BY-2悬浮细胞的凋亡[J].首都师范大学学报(自然科学版),2009,30(3):30-32. |
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| 作者姓名： | 韩利波 梁蒙 李佳雯 陈志玲 |
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| 作者单位： | 首都师范大学生命科学学院,北京,100048 |
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| 摘 要： | 烟草BY-2悬浮细胞经不同浓度的DMSO处理后发现，2％DMSO处理可导致细胞核染色质凝集或核结构解体，琼脂糖凝胶电泳显示基因组DNA降解成明显的梯状条带，从而表现出典型的细胞凋亡特征．对微丝骨架的进一步观察发现，2％DMSO处理引起细胞内微丝骨架分布异常，造成微丝骨架不同程度地断裂．这些结果为进一步研究微丝骨架在植物细胞凋亡中的功能奠定了基础．
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| 关 键 词： | 烟草悬浮细胞 凋亡 DMSO |
DMSO Induces Apoptosis of Tobacco Suspension BY-2 Cells |
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| Han Libo,Hang Meng,Li Jiawen,Chen Zhiling.DMSO Induces Apoptosis of Tobacco Suspension BY-2 Cells[J].Journal of Capital Normal University(Natural Science Edition),2009,30(3):30-32. |
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| Authors: | Han Libo Hang Meng Li Jiawen Chen Zhiling |
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| Institution: | College of Life Science;Capital Normal University;Beijing 100048 |
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| Abstract: | The tobacco suspension cells were treated with different concentrations of DMSO,then were labeled with fluorescent probe and analyzed with agarose electrophoresis.The results showed that 2% DMSO caused chromatin condensation,cytoplasm shrinkage and cell nucleus disintegration;the genomic DNA became fragment at some degree in apoptosis cells detected from agarose electrophoresis,which show the typical characteristic features of the apoptosis cells.In addition,microfilaments cleavaged into short fragments in ... |
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| Keywords: | DMSO |
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