Rapid purification of lactate dehydrogenase X from mouse testes by two steps of affinity chromatography on oxamate-sepharose |
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Authors: | H Spielmann H G Eibs C Mentzel |
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Institution: | (1) Pharmakologisches Institut, Abteilung Embryonal-Pharmakologie, Freie Universität, Thielallee 69-73, D-1 Berlin 33, (German Federal Republic, BRD) |
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Abstract: | Summary At concentrations of 200 M NADH and 0.5M NaCl LDH-X is separated from the other LDH isozymes of mouse testes on oxamate-sepharose. In a second step LDH-X is bound to the same matrix at lower NADH and NaCl concentrations and the pure enzyme can subsequently be eluted.Acknowledgment. This work was supported by grants of the Deutsche Forschungsgemeinschaft awarded to the Sonderforschungsbereich 29 Embryonale Differenzierung und Entwicklung. |
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