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半知菌曲霉族真菌胞外多糖的研究Ⅱ:爪哇正青霉胞外多糖化学结构的研究
引用本文:陈有为,李绍兰,杨丽源,黄浩海,李治滢,周斌.半知菌曲霉族真菌胞外多糖的研究Ⅱ:爪哇正青霉胞外多糖化学结构的研究[J].云南大学学报(自然科学版),2002,24(6):462-464.
作者姓名:陈有为  李绍兰  杨丽源  黄浩海  李治滢  周斌
作者单位:云南大学云南省微生物研究所,教育部微生物资源开放研究重点实验室,云南,昆明,650091
基金项目:云南省工业微生物发酵重点实验室基金项目 (2 0 0 0KF -0 0 5 )
摘    要: 经形态鉴定和18srRNA基因组序列分析,编号Ym31794菌株的分类学地位属于半知菌类丛梗孢科曲霉族青霉属丝状真菌,种名爪哇正青霉(Eupenicillium javanicum).该菌发酵液经离心弃菌体,除脂类及乙醇分级提取,分离、纯化的胞外多糖相对分子量为2.6×104,糖的质量分数为89%.真菌胞外多糖经红外光谱(IR),簿层层析(TLC),色质联用(GC-MS),核磁共振(13C-NMR,1H-NMR)等分析可知爪哇正青霉菌胞外多糖为1-6连接的葡萄糖和1-2连接半乳糖构成主干,侧链由1-3连接的甘露糖和1-2连接的葡萄糖构成,结构应为吡喃糖.

关 键 词:爪哇正青霉  18srDNA  胞外多糖化学结构
文章编号:0258-7971(2002)06-0462-03
修稿时间:2002年4月30日

A study on extracellular polysaccharides from Aspergileae of fungi imperfecti Ⅱ:chemical structure of extracellular polysaccharides from Eupenicillium javanicum
CHEN You wei,LI Shao lan,YANG Li yuang,HUANG Hao hai,LI Zhi ying,ZHOU Bin.A study on extracellular polysaccharides from Aspergileae of fungi imperfecti Ⅱ:chemical structure of extracellular polysaccharides from Eupenicillium javanicum[J].Journal of Yunnan University(Natural Sciences),2002,24(6):462-464.
Authors:CHEN You wei  LI Shao lan  YANG Li yuang  HUANG Hao hai  LI Zhi ying  ZHOU Bin
Institution:Yunnan Institute of Microbiology, Yunnan University, The Key Laboratory for Microbial Resources of Ministry of Education, Kunming 650091, China
Abstract:A strain YM31794,producing extracellular polysaccharide,was identified,using the methods of morphology and 18s rDNA sequencing analysis,as Eupenicillium javanicum Thom.The chemical structure of the extracellular polysaccharide from this strain was analysed through the reaction of methyl,Smith's degradation,thin layer chromatography,GC MS chromatography,IR spectrum, 13 C NMR and 1H NMR.The result indicate that this polysaccharide is a pyranose which is composed of the 1,6 bond linked glucose,1,2 bond linked galactose and 1,3 bond linked mannose ,and 1,2 bond linked glucose on its side chain.
Keywords:Eupenicillium javanicum  18s rDNA  chemical structure of polysaccharide
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