| 腺病毒介导人MDA-7/IL-24基因对人鼻咽癌细胞CNE的凋亡作用 |
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| 引用本文： | 苑兴卉,俞一鸣,王筱靓,王薇,黄青山.腺病毒介导人MDA-7/IL-24基因对人鼻咽癌细胞CNE的凋亡作用[J].复旦学报(自然科学版),2009,48(3). |
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| 作者姓名： | 苑兴卉 俞一鸣 王筱靓 王薇 黄青山 |
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| 作者单位： | 复旦大学,生命科学学院,遗传学研究所,遗传工程国家重点实验室,上海200433
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| 摘 要： | mda-7(melanoma differentiaton-associated gene 7,黑色素瘤分化相关基因7),其表达产物又称 IL-24(Interleukin-24,白介素24),现有实验证明其对多种肿瘤细胞有明显的生长抑制和凋亡诱导作用,具有广泛的癌症治疗前景和预期.鼻咽癌作为中国南方多发的癌症,目前少有mda-7对其凋亡作用的研究,本实验利用腺病毒载体(复制缺陷型)构建搭载mda-7的重组病毒Ad-mda7及空病毒Ad-GFP,用HEK 293T细胞对病毒进行扩增,得到的重组病毒用于人鼻咽癌细胞CNE的凋亡研究.Hoechst 33258凋亡染色以及流式细胞仪PI染色检测结果表明:重组病毒Ad-mda7相比空病毒Ad-GFP以及PBS处理组,对CNE细胞有较明显的凋亡诱导作用.
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| 关 键 词： | 重组腺病毒 鼻咽癌 |
Adenovirus-mediated Human MDA-7/IL-24 Transfer Induces Apoptosis in Human Nasopharyngeal Carcinoma Cell CNE |
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| YUAN Xing-hui,YU Yi-ming,WANG Xiao-liang,WANG Wei,HUANG Qing-shan.Adenovirus-mediated Human MDA-7/IL-24 Transfer Induces Apoptosis in Human Nasopharyngeal Carcinoma Cell CNE[J].Journal of Fudan University(Natural Science),2009,48(3). |
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| Authors: | YUAN Xing-hui YU Yi-ming WANG Xiao-liang WANG Wei HUANG Qing-shan |
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| Institution: | State Key Laboratory of Genetic Engineering;Institute of Genetics;School of Life Sciences;Fudan University;Shanghai 200433;China |
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| Abstract: | The protein product of mda-7(melanoma differentiation-associated gene 7),also known as IL-24(Interleukin-24),has been demonstrated to be capable of inhibiting cancer cells' proliferation and inducing different kinds of cancer cells' apoptosis,and having an encouraging future for cancer therapy.But there is little research focusing on the apoptosis-inducing of mda-7 in nasopharyngeal carcinoma,a common cancer in the south of China.Here,the replication-defective adenoviruses were used to deliver and express m... |
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| Keywords: | mda-7 |
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