| 用电子能量消失谱法研究P.tinctorius—P.massoniana菌根 |
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| 引用本文： | 钱晓鸣,黄耀坚,I.Kottke. 用电子能量消失谱法研究P.tinctorius—P.massoniana菌根[J]. 厦门大学学报(自然科学版), 2001, 40(5): 1156-1162 |
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| 作者姓名： | 钱晓鸣 黄耀坚 I.Kottke |
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| 作者单位： | 1. 厦门大学生命科学学院,
2. Tübingen大学生物系菌物所, |
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| 基金项目： | 国家自然科学基金(39770004)、国家留学生回国人员基金和福建省自然科学基金(C97003)资助项目 |
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| 摘 要： | 在实验室条件下用12株外生菌根菌，对马尾松（Pinus massoniana)培养苗进行高效菌种的接种试验，筛选并证明分布于台湾的采色豆马勃（Pisolithus tinctorius)，具有生长快，对生态环境要求不严格，接种成功率大的特点。用能量消失谱技术对细胞内N、P营养元素进行定位测定，表明了这一菌株具有对N、P元素的吸收和贮存能力。
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| 关 键 词： | 外生菌根 接种技术 能量消失谱技术 营养元素 氮 磷 马尾松 彩色豆马勃 |
| 文章编号： | 0438-0479(2001)05-1156-07 |
| 修稿时间： | 2001-01-05 |
Research on P. Tinctorius-P. Massoniana Mycorrhizae by Electron Energy-loss Spectroscopy |
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| QIAN Xiao ming ,HUANG Yao jian ,Ingrid Kottke. Research on P. Tinctorius-P. Massoniana Mycorrhizae by Electron Energy-loss Spectroscopy[J]. Journal of Xiamen University(Natural Science), 2001, 40(5): 1156-1162 |
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| Authors: | QIAN Xiao ming HUANG Yao jian Ingrid Kottke |
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| Affiliation: | QIAN Xiao ming 1,HUANG Yao jian 1,Ingrid Kottke 2 |
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| Abstract: | The mycorrhization of twelve mycorrhizal strains from Europe and Taiwan together with seedlings of Pinus massoniana Lamb. from Fujian was examined under vit ro-conditions. Among these strains Pisolithus tinctorius, originating from Taiwan, proved to be of easy mycorrhization, fast growing and insensitive to un favorable environmental conditions. The results of analysis of Pisolithus tinc torius-Pinus massoniana mycorrhizae by electron energy-loss spectroscopy(EEL S) showed this strain displayed a higher frequency of active hyphal sheaths and a high er potential to store nitrogen and phosphorus than other mycorrhizal types. Phos phorus and nitrogen were localized in vacuolar bodies.P. tinctorius was found to be more efficient in storing the macronutrient P and N. |
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| Keywords: | ectomycorrhizae inoculation electron energy- loss spectroscopy nutrient elements |
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