| 固定化细胞转化熊去氧胆酸 |
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| 引用本文： | 黄爱珉,周德庆.固定化细胞转化熊去氧胆酸[J].暨南大学学报,1994,15(3):94-98. |
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| 作者姓名： | 黄爱珉 周德庆 |
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| 作者单位： | 暨南大学生物系,复旦大学微生物学系 |
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| 基金项目： | 国家自然科学基金,暨南大学青年基金资助项目 |
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| 摘 要： |
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| 关 键 词： | 固定化细胞 熊去氧胆酸 生物转化 |
STUDY OF UDCA BIOTRANSFORMATION WITHIMMOBILIZED CELL OF ANMROBE |
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| Huang Aimin, Xie Shiqun,Zhou Deping,Hu Baolong.STUDY OF UDCA BIOTRANSFORMATION WITHIMMOBILIZED CELL OF ANMROBE[J].Journal of Jinan University(Natural Science & Medicine Edition),1994,15(3):94-98. |
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| Authors: | Huang Aimin Xie Shiqun Zhou Deping Hu Baolong |
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| Abstract: | Clostridium perfringens HS-10 was immobilized to study the biotransformation ofchenodeoxycholic acid(CDCA) to ursodeoxycholic acid in order to increase the substrateconcentration ,the anaerobe tolarence to substrate and the bioconversion rate, the iniinobilzedcells were made with ditferent adsorbing and embedding materials. It was found tbat calciumalginate, porous eramic and vermiculite were perfect immobilizing materials if the anaerobe HS -10 was "pre-cultured"in RCM medium containing 1.2mmol/L CDCA, the tolerence tosubstrate could be remarkably mised when the bacterial cells were embedded with 2% calciumalginate.The greatest conversion rates were 40% and 20% with substrate concentiations up to1.0mmol/L and 2.0 mmol/L. |
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| Keywords: | anaerobe immobilized cell ursodeoxycholic acid biotransformation |
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