| 转座子诱变甘蓝黑腐病菌所获胞外多糖突变体的类型 |
| |
| 引用本文： | 查冬兴,唐纪良,马庆生.转座子诱变甘蓝黑腐病菌所获胞外多糖突变体的类型[J].广西科学,1997,4(1):34-37. |
| |
| 作者姓名： | 查冬兴 唐纪良 马庆生 |
| |
| 作者单位： | 广西农业大学分子遗传研究室, 南宁市秀灵路13号 530005,广西农业大学分子遗传研究室, 南宁市秀灵路13号 530005,广西农业大学分子遗传研究室, 南宁市秀灵路13号 530005 |
| |
| 基金项目： | 国家自然科学基金资助项目。(项目编号39100064)。 |
| |
| 摘 要： | 据对转转子Ｔｎ５ｇｕｓＡ５诱变甘蓝黑腐病民获得３９株胞外多糖突变休的３种胞外酶活性和在含２％葡萄ＮＹＧＡ培养基上的菌落形态的检测结果，将突变株分成５种类型。Ｓｏｕｔｈｅｒｎ杂交结果表明它们的突变位于基因组中的８个不同的位置，其中１个位于已鉴定的ｒｐｆ调控基因簇。
|
| 关 键 词： | 甘蓝黑腐病菌 胞外多糖 突变体 |
| 收稿时间： | 1996/11/12 0:00:00 |
| 修稿时间： | 1996/12/13 0:00:00 |
Classification of the Exopolysaccharide-deficient Mutants of Xanthomonas campestris pv. campestris Derived from Transposon Mutagenesis |
| |
| Zha Dongxing,Tang Jiliang and Ma Qingsheng.Classification of the Exopolysaccharide-deficient Mutants of Xanthomonas campestris pv. campestris Derived from Transposon Mutagenesis[J].Guangxi Sciences,1997,4(1):34-37. |
| |
| Authors: | Zha Dongxing Tang Jiliang and Ma Qingsheng |
| |
| Institution: | Laboratory of Molecular Genetics, Guangxi Agricultural University, 13 Xuiling Road, Nanning, Guangxi, 530005,Laboratory of Molecular Genetics, Guangxi Agricultural University, 13 Xuiling Road, Nanning, Guangxi, 530005 and Laboratory of Molecular Genetics, Guangxi Agricultural University, 13 Xuiling Road, Nanning, Guangxi, 530005 |
| |
| Abstract: | The 39 exopolysaccharide-deficient mutants of Xanthomonas campestris pv. campestris, which were derived from the transposon Tn5gusA5 mutagenesis, were divided into five groups by their activities of three extracellular enzymes and colony-phenotypes on the NYGA plates containing 2% glucose. Southern hybridization analysis revealed that their mutation sites were located respectively in eight different positions of the genome and one of them was located in the identified rpf regulatory gene cluster. |
| |
| Keywords: | Xanthomonas campestris pv campestris exopolysaccharide mutant |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《广西科学》浏览原始摘要信息 |
| 点击此处可从《广西科学》下载免费的PDF全文 |
|
